ArticleBiophysical journal1991
Nanosecond fluorescence microscopy. Emission kinetics of fura-2 in single cells.
Article in Biophysical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Fluorescence lifetime excitation cytometry by kinetic dithering.Electrophoresis · 2014Article
- Time-resolved polarization imaging by pump-probe (stimulated emission) fluorescence microscopy.Biophysical journal · 2000Article
- Calcium transients and the effect of a photolytically released calcium chelator during electrically induced contractions in rabbit rectococcygeus smooth muscle.Biophysical journal · 1998Article
- Fluorescence lifetime imaging by asynchronous pump-probe microscopy.Biophysical journal · 1995Article
- Possibility of simultaneously measuring low and high calcium concentrations using Fura-2 and lifetime-based sensing.Cell calcium · 1995Article
- Fluorescence lifetime-based biosensing of zinc: Origin of the broad dynamic range.Journal of fluorescence · 1995Article
- Photophysics of the fluorescent Ca2+ indicator Fura-2.Biophysical journal · 1995Article
- Pulsed laser imaging of rapid Ca2+ gradients in excitable cells.Biophysical journal · 1994Article
- Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin-2.Cell calcium · 1994Article
- Fluorescence lifetime imaging microscopy: homodyne technique using high-speed gated image intensifier.Methods in enzymology · 1994Article
- Fluorescence anisotropy imaging microscopy maps calmodulin binding during cellular contraction and locomotion.The Journal of cell biology · 1993Article
- Phase Fluorometry Using a Continuously Modulated Laser Diode.Analytical chemistry · 1992Article
- Basal Ca2+ and the oscillation of Ca2+ in caffeine-treated bullfrog sympathetic neurones.The Journal of physiology · 1992Article
- Calcium imaging using fluorescence lifetimes and long-wavelength probes.Journal of fluorescence · 1992Article
- Fluorescence lifetime imaging of calcium using Quin-2.Cell calcium · 1992Article
- Fluorescence lifetime imaging of free and protein-bound NADH.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Thin-section ratiometric Ca2+ images obtained by optical sectioning of fura-2 loaded mast cells.The Journal of cell biology · 1992Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
A microscope based time-correlated single photon counting instrument has been constructed to measure fluorescence intensity and emission anisotropy decays from fluorophores in single cells on a nanosecond time scale. The sample is excited and the emission collected using epi-illumination optics with frequency-doubled pulses from the cavity-dumped output of a synchronously pumped dye laser serving as an excitation source. Collection of decays from a single cell is possible due to the presence of an iris in the emission path that can be reduced to less than the diameter of a single cell. Using the instrument the decay of 60 nM 1,6-diphenyl-1,3,5-hexatriene was measured, demonstrating that adequate data for lifetime analysis can be recorded from fewer 10(3) molecules of the fluorophore in an illuminated volume of 23 fl. In addition, the intensity and anisotropy decays of fura-2 in single adherent cells and in suspensions of fura-2 loaded cells in suspension, although the relative amplitudes and decay constants vary somewhat from cell to cell. The results indicate that a significant but variable fraction of fura-2 is bound to relatively immobile macromolecular components in these cells.
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