ArticleMatrix biology : journal of the International Society for Matrix Biology2010
Adult equine bone marrow stromal cells produce a cartilage-like ECM mechanically superior to animal-matched adult chondrocytes.
Article in Matrix biology : journal of the International Society for Matrix Biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
- Nanomechanics of Aggrecan: A New Perspective on Cartilage Biomechanics, Disease and Regeneration.Advances in experimental medicine and biology · 2023Article
- Impaired bone healing by enoxaparin via inhibiting the differentiation of bone marrow mesenchymal stem cells towards osteoblasts.Journal of bone and mineral metabolism · 2022Article
- Aggrecan: Approaches to Study Biophysical and Biomechanical Properties.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Mechanical Heterogeneity in the Bone Microenvironment as Characterized by Atomic Force Microscopy.Biophysical journal · 2020Article
- Gene expression profiling of progenitor cells isolated from rat rotator cuff musculotendinous junction.BMC musculoskeletal disorders · 2020Article
- Enzyme Pretreatment plus Locally Delivered HB-IGF-1 Stimulate Integrative Cartilage RepairTissue engineering. Part A · 2019Article
- Differences in the intrinsic chondrogenic potential of equine umbilical cord matrix and cord blood mesenchymal stromal/stem cells for cartilage regeneration.Scientific reports · 2018Article
- Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy.International journal of molecular sciences · 2018Article
- Repair of Osteochondral Defects in a Rabbit Model Using Bilayer Poly(Lactide-co-Glycolide) Scaffolds Loaded with Autologous Platelet-Rich Plasma.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- AFM-Nanomechanical Test: An Interdisciplinary Tool That Links the Understanding of Cartilage and Meniscus Biomechanics, Osteoarthritis Degeneration, and Tissue Engineering.ACS biomaterials science & engineering · 2017Article
- Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment.Tissue engineering. Part A · 2016Article
- Bioluminescence-mediated longitudinal monitoring of adipose-derived stem cells in a large mammal ex vivo organ culture.Scientific reports · 2015Article
- Delivering heparin-binding insulin-like growth factor 1 with self-assembling peptide hydrogels.Tissue engineering. Part A · 2015Article
- Dynamic nanomechanics of individual bone marrow stromal cells and cell-matrix composites during chondrogenic differentiation.Journal of biomechanics · 2015Article
- Sustained delivery of bioactive TGF-β1 from self-assembling peptide hydrogels induces chondrogenesis of encapsulated bone marrow stromal cells.Journal of biomedical materials research. Part A · 2014Article
- Advancing musculoskeletal research with nanoscience.Nature reviews. Rheumatology · 2013Review
- Age-related nanostructural and nanomechanical changes of individual human cartilage aggrecan monomers and their glycosaminoglycan side chains.Journal of structural biology · 2013Article
- Article
- Time-dependent processes in stem cell-based tissue engineering of articular cartilage.Stem cell reviews and reports · 2012Article
- Cartilage matrix formation by bovine mesenchymal stem cells in three-dimensional culture is age-dependent.Clinical orthopaedics and related research · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Our objective was to evaluate the age-dependent mechanical phenotype of bone marrow stromal cell- (BMSC-) and chondrocyte-produced cartilage-like neo-tissue and to elucidate the matrix-associated mechanisms which generate this phenotype. Cells from both immature (2-4 month-old foals) and skeletally-mature (2-5 year-old adults) mixed-breed horses were isolated from animal-matched bone marrow and cartilage tissue, encapsulated in self-assembling-peptide hydrogels, and cultured with and without TGF-beta1 supplementation. BMSCs and chondrocytes from both donor ages were encapsulated with high viability. BMSCs from both ages produced neo-tissue with higher mechanical stiffness than that produced by either young or adult chondrocytes. Young, but not adult, chondrocytes proliferated in response to TGF-beta1 while BMSCs from both age groups proliferated with TGF-beta1. Young chondrocytes stimulated by TGF-beta1 accumulated ECM with 10-fold higher sulfated-glycosaminoglycan content than adult chondrocytes and 2-3-fold higher than BMSCs of either age. The opposite trend was observed for hydroxyproline content, with BMSCs accumulating 2-3-fold more than chondrocytes, independent of age. Size-exclusion chromatography of extracted proteoglycans showed that an aggrecan-like peak was the predominant sulfated proteoglycan for all cell types. Direct measurement of aggrecan core protein length and chondroitin sulfate chain length by single molecule atomic force microscopy imaging revealed that, independent of age, BMSCs produced longer core protein and longer chondroitin sulfate chains, and fewer short core protein molecules than chondrocytes, suggesting that the BMSC-produced aggrecan has a phenotype more characteristic of young tissue than chondrocyte-produced aggrecan. Aggrecan ultrastructure, ECM composition, and cellular proliferation combine to suggest a mechanism by which BMSCs produce a superior cartilage-like neo-tissue than either young or adult chondrocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.