ArticleJournal of controlled release : official journal of the Controlled Release Society2010
Fibrin-mediated lentivirus gene transfer: implications for lentivirus microarrays.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Advances in viral vector-based delivery systems for gene therapy: a comprehensive review.3 Biotech · 2025Review
- A knock down strategy for rapid, generic, and versatile modelling of muscular dystrophies in 3D-tissue-engineered-skeletal muscle.Skeletal muscle · 2024Article
- Review
- Microgels produced using microfluidic on-chip polymer blending for controlled released of VEGF encoding lentivectors.Acta biomaterialia · 2018Article
- Biomaterial-Guided Gene Delivery for Musculoskeletal Tissue Repair.Tissue engineering. Part B, Reviews · 2017Review
- Localized lentivirus delivery via peptide interactions.Biotechnology and bioengineering · 2016Article
- Laminin-111 Peptides Conjugated to Fibrin Hydrogels Promote Formation of Lumen Containing Parotid Gland Cell Clusters.Biomacromolecules · 2016Article
- CDH2 and CDH11 act as regulators of stem cell fate decisions.Stem cell research · 2015Review
- Article
- Differential and synergistic effects of mechanical stimulation and growth factor presentation on vascular wall function.Biomaterials · 2013Article
- Growth factors polymerized within fibrin hydrogel promote amylase production in parotid cells.Tissue engineering. Part A · 2013Article
- Matrix-based gene delivery for tissue repair.Current opinion in biotechnology · 2013Review
- Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Sustained, localized transgene expression mediated from lentivirus-loaded biodegradable polyester elastomers.Journal of biomedical materials research. Part A · 2013Article
- Hydrogels for lentiviral gene delivery.Expert opinion on drug delivery · 2013Review
- Fibrin hydrogels for lentiviral gene delivery in vitro and in vivo.Journal of controlled release : official journal of the Controlled Release Society · 2012Article
- Engineering fibrin-binding TGF-β1 for sustained signaling and contractile function of MSC based vascular constructs.Biomaterials · 2011Article
- Engineering biomaterial systems to enhance viral vector gene delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Vascularization of the dermal support enhances wound re-epithelialization by in situ delivery of epidermal keratinocytes.Tissue engineering. Part A · 2011Article
- Hydrogels to modulate lentivirus delivery in vivo from microporous tissue engineering scaffolds.Drug delivery and translational research · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We employed fibrin hydrogel as a bioactive matrix for lentivirus mediated gene transfer. Fibrin-mediated gene transfer was highly efficient and exhibited strong dependence on fibrinogen concentration. Efficient gene transfer was achieved with fibrinogen concentration between 3.75 and 7.5mg/ml. Lower fibrinogen concentrations resulted in diffusion of virus out of the gel while higher concentrations led to ineffective fibrin degradation by target cells. Addition of fibrinolytic inhibitors decreased gene transfer in a dose-dependent manner suggesting that fibrin degradation by target cells may be necessary for successful gene delivery. Under these conditions transduction may be limited only to cells interacting with the matrix thereby providing a method for spatially-localized gene delivery. Indeed, when lentivirus-containing fibrin microgels were spotted in an array format gene transfer was confined to virus-containing fibrin spots with minimal cross-contamination between neighboring sites. Collectively, our data suggest that fibrin may provide an effective matrix for spatially-localized gene delivery with potential applications in high-throughput lentiviral microarrays and in regenerative medicine.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.