ArticleJournal of virological methods2010
A novel multiplex real-time RT-PCR assay with FRET hybridization probes for the detection and quantitation of 13 respiratory viruses.
Article in Journal of virological methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Review of air disinfection approaches and proposal for thermal inactivation of airborne viruses as a life-style and an instrument to fight pandemics.Applied thermal engineering · 2022Review
- Application of Nanoscale Materials and Nanotechnology Against Viral Infection: A Special Focus on Coronaviruses.Advances in experimental medicine and biology · 2021Article
- SARS-CoV-2 viral load assessment in respiratory samples.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
- Sequencing and analysis of globally obtained human parainfluenza viruses 1 and 3 genomes.PloS one · 2019Article
- Replacement of neuraminidase inhibitor-susceptible influenza A(H1N1) with resistant phenotype in 2008 and circulation of susceptible influenza A and B viruses during 2009-2013, South Africa.Influenza and other respiratory viruses · 2019Article
- Human bocavirus: Current knowledge and future challenges.World journal of gastroenterology · 2016Review
- The role of influenza, RSV and other common respiratory viruses in severe acute respiratory infections and influenza-like illness in a population with a high HIV sero-prevalence, South Africa 2012-2015.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2016Article
- Lung Infection by Human Bocavirus Induces the Release of Profibrotic Mediator Cytokines In Vivo and In Vitro.PloS one · 2016Article
- Viral and bacterial etiology of severe acute respiratory illness among children < 5 years of age without influenza in Niger.BMC infectious diseases · 2015Article
- Acute human bocavirus infection in MDS patient, Cologne, Germany.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2015Article
- Development and laboratory evaluation of a real-time PCR assay for detecting viruses and bacteria of relevance for community-acquired pneumonia.The Journal of molecular diagnostics : JMD · 2015Article
- Molecular diagnosis of respiratory viruses.Pathology · 2015Review
- Comparison of xTAG respiratory virus panel and Verigene Respiratory Virus Plus for detecting influenza virus and respiratory syncytial virus.Journal of clinical laboratory analysis · 2015Article
- The clinical and etiological characteristics of influenza-like illness (ILI) in outpatients in Shanghai, China, 2011 to 2013.PloS one · 2015Article
- Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection.Memorias do Instituto Oswaldo Cruz · 2014Article
- New molecular virus detection methods and their clinical value in lower respiratory tract infections in children.Paediatric respiratory reviews · 2013Review
- Human bocavirus: lessons learned to date.Pathogens (Basel, Switzerland) · 2013Review
- Influenza surveillance among children with pneumonia admitted to a district hospital in coastal Kenya, 2007-2010.The Journal of infectious diseases · 2012Article
- Respiratory viral infections during the 2009-2010 winter season in Central England, UK: incidence and patterns of multiple virus co-infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Comparison of two broadly multiplexed PCR systems for viral detection in clinical respiratory tract specimens from immunocompromised children.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative multiplex real-time RT-PCR assays utilizing fluorescence resonance energy transfer (FRET) hybridization probes were developed for the detection of 13 respiratory viruses, including well recognized viral causes (respiratory syncytial virus, influenza viruses A and B, parainfluenza viruses types 1, 2, and 3, adenovirus) as well as viruses described recently as causes of acute respiratory tract infections (human coronaviruses NL63, HKU1, 229E, and OC43, human bocavirus, and human metapneumovirus). FRET probes have an improved toleration for single base mismatches than other probe chemistries, reducing the chances of missing highly variable RNA viruses. The assay could detect 2.5-25 DNA/RNA copies/microl (2.5 x 10(3)-2.5 x 10(4) copies/ml). Validation on 91 known positive respiratory specimens indicated similar specificity as commercial direct immunofluorescence assays (IFA) or single-round PCRs used in initial identification. Screening of 270 IFA negative respiratory specimens identified new viruses in 40/270 (14.8%) cases and additional 79/270 (29.3%) well recognized viruses missed by routine diagnostic assays including 6.7% co-infections. All viruses could be detected in the clinical screening panel. The assays demonstrates an improved sensitivity and scope of detecting respiratory viruses relative to routine antigen detection assays while the quantitative utility may facilitate investigation of the role of co-infections and viral load in respiratory virus pathogenesis.
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