ArticleFEBS letters2010
Design and characterization of a constitutively open KcsA.
Article in FEBS letters, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Full opening of helix bundle crossing does not lead to NaK channel activation.The Journal of general physiology · 2022Article
- Article
- Lipid Membrane Mimetics in Functional and Structural Studies of Integral Membrane Proteins.Membranes · 2021Review
- Article
- Probing allosteric coupling in a constitutively open mutant of the ion channel KcsA using solid-state NMR.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The influence of membrane bilayer thickness on KcsA channel activity.Channels (Austin, Tex.) · 2019Article
- Conformational heterogeneity in closed and open states of the KcsA potassium channel in lipid bicelles.The Journal of general physiology · 2016Article
- Ion-binding properties of a K+ channel selectivity filter in different conformations.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The Hydrophobic Effect Contributes to the Closed State of a Simplified Ion Channel through a Conserved Hydrophobic Patch at the Pore-Helix Crossing.Frontiers in pharmacology · 2015Article
- Regulation of ion channel function by the host lipid bilayer examined by a stopped-flow spectrofluorometric assay.Biophysical journal · 2014Article
- Molecular interactions involved in proton-dependent gating in KcsA potassium channels.The Journal of general physiology · 2013Article
- Importance of lipid-pore loop interface for potassium channel structure and function.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Targeted molecular dynamics (TMD) of the full-length KcsA potassium channel: on the role of the cytoplasmic domain in the opening process.Journal of molecular modeling · 2013Article
- Role of the KcsA channel cytoplasmic domain in pH-dependent gating.Biophysical journal · 2011Article
- Mechanism of activation gating in the full-length KcsA K+ channel.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Bridging the gap between structural models of nicotinic receptor superfamily ion channels and their corresponding functional states.Journal of molecular biology · 2010Article
- Article
- A molecular mechanism for proton-dependent gating in KcsA.FEBS letters · 2010Article
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Authors and funding
7 authors.
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Abstract
The molecular nature of the structure responsible for proton sensitivity in KcsA has been identified as a charge cluster that surrounds the inner helical bundle gate. Here, we show that this proton sensor can be modified to engineer a constitutively open form of KcsA, amenable to functional, spectroscopic and structural analyses. By combining charge neutralizations for all acidic and basic residues in the cluster at positions 25, 117-122 and 124 (but not E118), a mutant KcsA is generated that displays constitutively open channel activity up to pH 9. The structure of this mutant revealed that full opening appears to be inhibited by lattice forces since the activation gate seems to be only on the early stages of opening.
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