ArticleAnalytical biochemistry2010
Development of a noncompetitive phage anti-immunocomplex assay for brominated diphenyl ether 47.
Article in Analytical biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Review
- Orientational binding modes of reporters in a viral-nanoparticle lateral flow assay.The Analyst · 2016Article
- Aptamer-Phage Reporters for Ultrasensitive Lateral Flow Assays.Analytical chemistry · 2015Article
- Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin.Analytica chimica acta · 2015Article
- Detection of viruses by counting single fluorescent genetically biotinylated reporter immunophage using a lateral flow assay.ACS applied materials & interfaces · 2015Article
- Recombinant streptavidin nanopeptamer anti-immunocomplex assay for noncompetitive detection of small analytes.Analytical chemistry · 2014Article
- Ultrasensitive immuno-detection using viral nanoparticles with modular assembly using genetically-directed biotinylation.Biotechnology letters · 2014Article
- Enhanced sensitive immunoassay: noncompetitive phage anti-immune complex assay for the determination of malachite green and leucomalachite green.Journal of agricultural and food chemistry · 2014Article
- Virus outbreaks in chemical and biological sensors.Sensors (Basel, Switzerland) · 2014Review
- Shiga-like toxin B subunit of Escherichia coli as scaffold for high-avidity display of anti-immunocomplex peptides.Analytical chemistry · 2014Article
- Nanopeptamers for the development of small-analyte lateral flow tests with a positive readout.Analytical chemistry · 2013Article
- Noncompetitive phage anti-immunocomplex real-time polymerase chain reaction for sensitive detection of small molecules.Analytical chemistry · 2011Article
- Phage anti-immunocomplex assay for clomazone: two-site recognition increasing assay specificity and facilitating adaptation into an on-site format.Analytical chemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
We present a new application of the noncompetitive phage anti-immunocomplex assay (PHAIA) by converting an existing competitive assay to a versatile noncompetitive sandwich-type format using immunocomplex binding phage-borne peptides to detect the brominated flame retardant, brominated diphenyl ether 47 (BDE 47). Three phage-displayed 9-mer disulfide-constrained peptides that recognize the BDE 47-polyclonal antibody immunocomplex were isolated. The resulting PHAIAs showed variable sensitivities, and the most sensitive peptide had a dose-response curve with an SC(50) (concentration of analyte producing 50% saturation of the signal) of 0.7ng/ml BDE 47 and a linear range of 0.3-2ng/ml, which was nearly identical to the best heterologous competitive format (IC(50) of 1.8ng/ml, linear range of 0.4-8.5/ml). However, the PHAIA was 1400-fold better than homologous competitive assay. The validation of the PHAIA with extracts of house furniture foam as well as human and calf sera spiked with BDE 47 showed overall recovery of 80-113%. The PHAIA was adapted to a dipstick format (limit of detection of 3.0ng/ml), and a blind test with six random extracts of local house furniture foams showed that the results of the PHAIA and dipstick assay were consistent, giving the same positive and negative detection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.