ArticleStructure (London, England : 1993)2010
Insights from the structure of a smallpox virus topoisomerase-DNA transition state mimic.
Article in Structure (London, England : 1993), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Identification of Repurposed Drugs Against Monkeypox Virus Through Comprehensive Virtual Screening.Biotechnology journal · 2026Article
- Deletions initiated by the vaccinia virus TopIB protein in yeast.DNA repair · 2024Article
- Gel-Free Tools for Quick and Simple Screening of Anti-Topoisomerase 1 Compounds.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Anti-viral drug discovery against monkeypox and smallpox infection by natural curcumin derivatives: A Computational drug design approach.Frontiers in cellular and infection microbiology · 2023Article
- Topoisomerase I (TOP1) dynamics: conformational transition from open to closed states.Nature communications · 2022Article
- Article
- Characterization of DNA Binding by the Isolated N-Terminal Domain of Vaccinia Virus DNA Topoisomerase IB.Biochemistry · 2017Article
- Stereospecific suppression of active site mutants by methylphosphonate substituted substrates reveals the stereochemical course of site-specific DNA recombination.Nucleic acids research · 2015Article
- Biochemical characterization of the topoisomerase domain of Methanopyrus kandleri topoisomerase V.The Journal of biological chemistry · 2014Article
- Variola type IB DNA topoisomerase: DNA binding and supercoil unwinding using engineered DNA minicircles.Biochemistry · 2014Article
- Chemical mutagenesis of vaccinia DNA topoisomerase lysine 167 provides insights to the catalysis of DNA transesterification.Biochemistry · 2013Article
- Diverse energetic effects of charge reversal mutations of poxvirus topoisomerase IB.Biochemistry · 2012Article
- Topoisomerases and site-specific recombinases: similarities in structure and mechanism.Critical reviews in biochemistry and molecular biology · 2010Review
- Requirements for catalysis in the Cre recombinase active site.Nucleic acids research · 2010Article
- Review
- Crystal structure of a bacterial topoisomerase IB in complex with DNA reveals a secondary DNA binding site.Structure (London, England : 1993) · 2010Article
- Topoisomerase IB-DNA interactions: X marks the spot.Structure (London, England : 1993) · 2010Article
- Mechanism and specificity of DNA strand exchange catalyzed by vaccinia DNA topoisomerase type I.Biochemistry · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Poxviruses encode their own type IB topoisomerases (TopIBs), which release superhelical tension generated by replication and transcription of their genomes. To investigate the reaction catalyzed by viral TopIBs, we have determined the structure of a variola virus topoisomerase-DNA complex trapped as a vanadate transition state mimic. The structure reveals how the viral TopIB enzymes are likely to position the DNA duplex for ligation following relaxation of supercoils and identifies the sources of friction observed in single-molecule experiments that argue against free rotation. The structure also identifies a conformational change in the leaving group sugar that must occur prior to cleavage and reveals a mechanism for promoting ligation following relaxation of supercoils that involves an Asp-minor groove interaction. Overall, the new structural data support a common catalytic mechanism for the TopIB superfamily but indicate distinct methods for controlling duplex rotation in the small versus large enzyme subfamilies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.