ArticleInternational journal of biological sciences2010
Overexpression of bacterial ethylene-forming enzyme gene in Trichoderma reesei enhanced the production of ethylene.
Article in International journal of biological sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 54 citations in OpenAlex.
- Experimental Evidence for Radical-Polar Crossover in Competition with, Rather Than Preceding, Alkene Formation by Microbial Ethylene-Forming Enzyme.Journal of the American Chemical Society · 2025Article
- Research advances on the consolidated bioprocessing of lignocellulosic biomass.Engineering microbiology · 2024Review
- Identification and characterization of sugar-regulated promoters in Chaetomium thermophilum.BMC biotechnology · 2023Article
- Discovery of Five New Ethylene-Forming Enzymes for Clean Production of Ethylene inInternational journal of molecular sciences · 2022Article
- Metabolic engineering of Zymomonas moblis for ethylene production from straw hydrolysate.Applied microbiology and biotechnology · 2021Article
- Biochemical pathways to α-ketoglutarate, a multi-faceted metabolite.World journal of microbiology & biotechnology · 2020Review
- Metatranscriptomics and Amplicon Sequencing Reveal Mutualisms in Seagrass Microbiomes.Frontiers in microbiology · 2018Article
- Bio-production of gaseous alkenes: ethylene, isoprene, isobutene.Biotechnology for biofuels · 2018Review
- Ethylene production with engineered Synechocystis sp PCC 6803 strains.Microbial cell factories · 2017Article
- Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei.Microbial cell factories · 2016Review
- Comparative sequence analysis and mutagenesis of ethylene forming enzyme (EFE) 2-oxoglutarate/Fe(II)-dependent dioxygenase homologs.BMC biochemistry · 2014Article
- Genome organization and assessment of high copy number and increased expression of pectinolytic genes from Penicillium griseoroseum: a potential heterologous system for protein production.Journal of industrial microbiology & biotechnology · 2014Article
- Ethylene-forming enzyme and bioethylene production.Biotechnology for biofuels · 2014Article
- Identification of factors for improved ethylene production via the ethylene forming enzyme in chemostat cultures of Saccharomyces cerevisiae.Microbial cell factories · 2013Article
- Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to efficiently utilize natural cellulose materials to produce ethylene, three expression vectors containing the ethylene-forming enzyme (efe) gene from Pseudomonas syringae pv. glycinea were constructed. The target gene was respectively controlled by different promoters: cbh I promoter from Trichoderma reesei cellobiohydrolases I gene, gpd promoter from Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase gene and pgk I promoter from T. reesei 3-phosphoglycerate kinase I gene. After transforming into T. reesei QM9414, 43 stable transformants were obtained by PCR amplification and ethylene determination. Southern blot analysis of 14 transformants demonstrated that the efe gene was integrated into chromosomal DNA with copy numbers from 1 to 4. Reverse transcription polymerase chain reaction (RT-PCR) analysis of 6 transformants showed that the heterologous gene was transcribed. By using wheat straw as a carbon source, the ethylene production rates of aforementioned 14 transformants were measured. Transformant C30-3 with pgk I promoter had the highest ethylene production (4,012 nl h(-1) l(-1)). This indicates that agricultural wastes could be used to produce ethylene in recombinant filamentous fungus T. reesei.
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