ArticleJournal of the American Society of Nephrology : JASN2010
Integrative urinary peptidomics in renal transplantation identifies biomarkers for acute rejection.
Article in Journal of the American Society of Nephrology : JASN, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03317925 (Renal Transplant Injury and the Renin-Angiotensin System in Kids), which is not on this map. Cited by 61 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Renal Transplant Injury and the Renin-Angiotensin System in Kids (RETASK)
Who cites it
61 citing papers in PubMed, 1 synthesis or guideline pooled it, 127 citations in OpenAlex.
- A Computational Gene Expression Score for Predicting Immune Injury in Renal Allografts.PloS one · 2015Pooled it
- Non-invasive diagnosis of acute rejection in renal transplant patients using mass spectrometry of urine samples - a multicentre phase 3 diagnostic accuracy study.BMC nephrology · 2015Trial
- Trial
- A rapid noninvasive assay for the detection of renal transplant injury.Transplantation · 2013Trial
- Review
- Matrix metalloproteinases in kidney homeostasis and diseases: an update.American journal of physiology. Renal physiology · 2024Review
- Recent progress in mass spectrometry-based urinary proteomics.Clinical proteomics · 2024Review
- Urine MMP7 as a kidney injury biomarker.Clinical kidney journal · 2024Article
- Renal Fibrosis in Lupus Nephritis.International journal of molecular sciences · 2022Review
- Proteomics for Biomarker Discovery for Diagnosis and Prognosis of Kidney Transplantation Rejection.Proteomes · 2022Review
- Noninvasive Diagnosis of Acute Rejection in Renal Transplant Patients Using Mass Spectrometric Analysis of Urine Samples: A Multicenter Diagnostic Phase III Trial.Transplantation direct · 2022Article
- Omics-based biomarkers for diagnosis and prediction of kidney allograft rejection.The Korean journal of internal medicine · 2022Review
- Progress in kidney transplantation: The role for systems immunology.Frontiers in medicine · 2022Review
- T Cells With ActivatedFrontiers in immunology · 2022Article
- Deep sequencing of DNA from urine of kidney allograft recipients to estimate donor/recipient-specific DNA fractions.PloS one · 2021Article
- Article
- Peptidomic Analysis of Urine from Youths with Early Type 1 Diabetes Reveals Novel Bioactivity of Uromodulin PeptidesMolecular & cellular proteomics : MCP · 2020Article
- Bioinformatics for Renal and Urinary Proteomics: Call for Aggrandization.International journal of molecular sciences · 2020Review
- The Yin and Yang of Alarmins in Regulation of Acute Kidney Injury.Frontiers in medicine · 2020Review
- Urinary Peptidomic Biomarkers in Kidney Diseases.International journal of molecular sciences · 2019Review
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Noninvasive methods to diagnose rejection of renal allografts are unavailable. Mass spectrometry followed by multiple-reaction monitoring provides a unique approach to identify disease-specific urine peptide biomarkers. Here, we performed urine peptidomic analysis of 70 unique samples from 50 renal transplant patients and 20 controls (n = 20), identifying a specific panel of 40 peptides for acute rejection (AR). Peptide sequencing revealed suggestive mechanisms of graft injury with roles for proteolytic degradation of uromodulin (UMOD) and several collagens, including COL1A2 and COL3A1. The 40-peptide panel discriminated AR in training (n = 46) and test (n = 24) sets (area under ROC curve >0.96). Integrative analysis of transcriptional signals from paired renal transplant biopsies, matched with the urine samples, revealed coordinated transcriptional changes for the corresponding genes in addition to dysregulation of extracellular matrix proteins in AR (MMP-7, SERPING1, and TIMP1). Quantitative PCR on an independent set of 34 transplant biopsies with and without AR validated coordinated changes in expression for the corresponding genes in rejection tissue. A six-gene biomarker panel (COL1A2, COL3A1, UMOD, MMP-7, SERPING1, TIMP1) classified AR with high specificity and sensitivity (area under ROC curve = 0.98). These data suggest that changes in collagen remodeling characterize AR and that detection of the corresponding proteolytic degradation products in urine provides a noninvasive diagnostic approach.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.