ArticleProceedings of the National Academy of Sciences of the United States of America2010
Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- Ergosterol from Yeasts PromotesInternational journal of molecular sciences · 2026Article
- Deep learning reveals endogenous sterols as allosteric modulators of the GPCR-Gα interface.eLife · 2025Article
- Targeted regulation of sterol biosynthesis genes according to perturbations in ergosterol biosynthesis in fungi.Journal of advanced research · 2025Article
- Raman Imaging of an Amphotericin B Potentiator in Single-CellThe journal of physical chemistry. B · 2025Article
- Article
- Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escin.Scientific reports · 2023Article
- A high-throughput RNA-Seq approach to elucidate the transcriptional response of Piriformospora indica to high salt stress.Scientific reports · 2021Article
- Understanding the Impact of Industrial Stress Conditions on Replicative Aging inFrontiers in fungal biology · 2021Review
- The Effects of Tormentic Acid and Extracts fromTheScientificWorldJournal · 2021Article
- Plasma Membrane Fusion Is Specifically Impacted by the Molecular Structure of Membrane Sterols During Vegetative Development ofGenetics · 2020Article
- The yeast cell wall protein Pry3 inhibits mating through highly conserved residues within the CAP domain.Biology open · 2020Article
- Rate-limiting steps in the Saccharomyces cerevisiae ergosterol pathway: towards improved ergosta-5,7-dien-3β-ol accumulation by metabolic engineering.World journal of microbiology & biotechnology · 2018Article
- From Lipid Homeostasis to Differentiation: Old and New Functions of the Zinc Cluster Proteins Ecm22, Upc2, Sut1 and Sut2.International journal of molecular sciences · 2017Review
- Accumulation of specific sterol precursors targets a MAP kinase cascade mediating cell-cell recognition and fusion.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Cell biology of yeast zygotes, from genesis to budding.Biochimica et biophysica acta · 2015Review
- Discovery of an ergosterol-signaling factor that regulates Trypanosoma brucei growth.Journal of lipid research · 2015Article
- Article
- Article
- Systematic lipidomic analysis of yeast protein kinase and phosphatase mutants reveals novel insights into regulation of lipid homeostasis.Molecular biology of the cell · 2014Article
- Membrane organization and cell fusion during mating in fission yeast requires multipass membrane protein Prm1.Genetics · 2014Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
Under mating conditions, yeast cells adopt a characteristic pear-shaped morphology, called a "shmoo," as they project a cell extension toward their mating partners. Mating partners make contact at their shmoo tips, dissolve the intervening cell wall, and fuse their plasma membranes. We identified mutations in ERG4, encoding the enzyme that catalyzes the last step of ergosterol biosynthesis, that impair both shmoo formation and cell fusion. Upon pheromone treatment, erg4Delta mutants polarized growth, lipids, and proteins involved in mating but did not form properly shaped shmoos and fused with low efficiency. Supplementation with ergosterol partially suppressed the shmooing defect but not the cell fusion defect. By contrast, removal of the Erg4 substrate ergosta-5,7,22,24(28)-tetraenol, which accumulates in erg4Delta mutant cells and contains an extra double bond in the aliphatic chain of the sterol, restored both shmooing and cell fusion to wild-type levels. Thus, a two-atom change in the aliphatic moiety of ergosterol is sufficient to obstruct cell shape remodeling and cell fusion.
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