ArticleDevelopment (Cambridge, England)2010
The bHLH/PAS transcription factor singleminded 2s promotes mammary gland lactogenic differentiation.
Article in Development (Cambridge, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Deciphering the regulatory role of ELF5 in buffalo lactation.Frontiers in veterinary science · 2025Article
- The role of bHLH-PAS transcription factors in endoplasmic reticulum stress.Frontiers in molecular biosciences · 2025Review
- SIM2s directed Parkin-mediated mitophagy promotes mammary epithelial cell differentiation.Cell death and differentiation · 2023Article
- Noncanonical role of singleminded-2s in mitochondrial respiratory chain formation in breast cancer.Experimental & molecular medicine · 2023Article
- TF-Prioritizer: a Java pipeline to prioritize condition-specific transcription factors.GigaScience · 2022Article
- Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.iScience · 2021Article
- Article
- Loss of SIM2s inhibits RAD51 binding and leads to unresolved replication stress.Breast cancer research : BCR · 2019Article
- In Vitro Models for Studying Invasive Transitions of Ductal Carcinoma In Situ.Journal of mammary gland biology and neoplasia · 2019Review
- Multiple roles of single-minded 2 in esophageal squamous cell carcinoma and its clinical implications.Cancer science · 2018Article
- Dioxin exposure blocks lactation through a direct effect on mammary epithelial cells mediated by the aryl hydrocarbon receptor repressor.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
- bHLH-PAS proteins in cancer.Nature reviews. Cancer · 2013Review
- Article
- Epigenetic modifications unlock the milk protein gene loci during mouse mammary gland development and differentiation.PloS one · 2013Article
- Sim2 prevents entry into the myogenic program by repressing MyoD transcription during limb embryonic myogenesis.Development (Cambridge, England) · 2012Article
- Molecular cloning and its expression of trachealess gene (As-trh) during development in brine shrimp, Artemia sinica.Molecular biology reports · 2012Article
- Singleminded-2s (Sim2s) promotes delayed involution of the mouse mammary gland through suppression of Stat3 and NFκB.Molecular endocrinology (Baltimore, Md.) · 2011Article
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
We have previously demonstrated that the bHLH/PAS transcription factor, singleminded 2s (Sim2s), is required for proper mammary ductal morphogenesis and luminal epithelial differentiation. Furthermore, loss of Sim2s in breast cancer cells resulted in downregulation of epithelial markers and acquisition of a basal-like phenotype. The objective of this study was to further define the role of Sim2s in mammary differentiation. We found that Sim2s is developmentally regulated throughout mammary gland development with highest expression during lactation. Mammary glands from nulliparous mice expressing Sim2s driven by the mouse mammary tumor virus (MMTV) long terminal repeat (LTR) promoter were morphologically indistinguishable from wild-type mice but displayed hallmarks of precocious lactogenic differentiation. These included elevated expression of the milk protein genes Wap and Csn2, and apical localization of the lactation marker Npt2b. Consistent with the in vivo results, Sim2s enhanced prolactin-mediated Csn2 expression in HC11 and CIT3 mouse mammary epithelial cells, and downregulation of Sim2s by shRNA in HC11 cells inhibited Csn2 expression. Chromatin immunoprecipitation (ChIP) analyses of the Csn2 gene found that Sim2s associates with the Csn2 promoter and re-ChIP experiments showed that Sim2s interacted with the RNA II polymerase (RNAPII) complex. Together, these data demonstrate, for the first time, that Sim2s is required for establishing and maintaining mammary gland differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.