ArticleJournal of immunological methods2010
Comparison of polyclonal expansion methods to improve the recovery of cervical cytobrush-derived T cells from the female genital tract of HIV-infected women.
Article in Journal of immunological methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Nonsurgical Induction of Alopecia Areata in C3H/HeJ Mice via Adoptive Transfer of Cultured Lymphoid Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- SMAC mimetics inhibit human T cell proliferation and fail to augment type 1 cytokine responses.Cellular immunology · 2023Article
- Challenges of CRISPR-Based Gene Editing in Primary T Cells.International journal of molecular sciences · 2022Review
- Preservation of antigen-specific responses in cryopreserved CD4Journal of translational autoimmunity · 2022Article
- Influence of Culture Conditions on Ex Vivo Expansion of T Lymphocytes and Their Function for Therapy: Current Insights and Open Questions.Frontiers in bioengineering and biotechnology · 2022Review
- Tissue issues: mucosal T-cell responses in HIV-1 infection.Current opinion in HIV and AIDS · 2019Review
- Impact of HIV-ART on the restoration of Th17 and Treg cells in blood and female genital mucosa.Scientific reports · 2019Article
- Mucosal Immunity in HIV/SIV Infection: T Cells, B Cells and Beyond.Current immunology reviews · 2019Article
- Characteristics of HIV target CD4 T cells collected using different sampling methods from the genital tract of HIV seronegative women.PloS one · 2017Article
- Persistence of mucosal T-cell responses to herpes simplex virus type 2 in the female genital tract.Mucosal immunology · 2015Article
- Intramuscular therapeutic vaccination targeting HPV16 induces T cell responses that localize in mucosal lesions.Science translational medicine · 2014Article
- Article
- HIV-specific T-cell responses detected in the genital tract of chronically HIV-infected women are largely monofunctional.Immunology · 2013Article
- Vaccine applications of flow cytometry.Methods (San Diego, Calif.) · 2012Review
- Stability and transport of cervical cytobrushes for isolation of mononuclear cells from the female genital tract.Journal of immunological methods · 2011Article
- Immune responses to HIV in the female reproductive tract, immunologic parallels with the gastrointestinal tract, and research implications.American journal of reproductive immunology (New York, N.Y. : 1989) · 2011Review
- Evaluation of a quantitative real-time PCR assay to measure HIV-specific mucosal CD8+ T cell responses in the cervix.PloS one · 2010Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Cervical cytobrushing is a useful and non-invasive method for obtaining mucosal mononuclear cells from the female genital tract, but yields few cells. The aim of this study was to compare in vitro expansion protocols (anti-CD3, anti-CD3/CD28 or Dynal anti-CD3/CD28 beads) and cytokine combinations (IL-2, IL-7 and IL-15) to improve cervical T cell yields and viability. Eighteen HIV-infected women were included in this study to compare methods for polyclonal expansion of T cells from the female genital tract and blood. Comparison of T cell yields, viability and maturational status (by differential staining with CD45RO, CCR7 and CD27) was determined following 7 days of in vitro expansion. Anti-CD3 and IL-2 resulted in a 4.5-fold (range 3.7-5.3) expansion of cervical CD3+ T cells in 7 days compared to day 0. Inclusion of anti-CD28 or addition of IL-7 and IL-15 to this combination did not improve expansion. Culturing cells with Dynal beads (1:1) and IL-2, IL-7 and IL-15 gave rise to the highest yields after 7 days in both blood (7.1-fold) and cervix (5.6-fold). While expansion with anti-CD3 led to the accumulation of effector memory T cells (CD45RO+CCR7-CD27-), expansion with Dynabeads selected for accumulation of central memory T cells (CD45RO+CCR7+CD27+). We conclude that in vitro expansion with Dynabeads (1:1) in the presence of IL-2, IL-7 and IL-15 resulted in the greatest increase in viable T cells from both blood and cytobrush. Irrespective of the expansion method used, the T cell memory profile was altered following expansion.
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