ArticleArthritis research & therapy2010
High-density lipoproteins downregulate CCL2 production in human fibroblast-like synoviocytes stimulated by urate crystals.
Article in Arthritis research & therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 65 citations in OpenAlex.
- The interplay between NLRP3 inflammasome and metabolic signals in gouty arthritis.Frontiers in immunology · 2026Review
- Article
- Association Between Triglyceride-Glucose Index and Gout in Young Adults in China.International journal of general medicine · 2025Article
- Truncating NFKB1 variants cause combined NLRP3 inflammasome activation and type I interferon signaling and predispose to necrotizing fasciitis.Cell reports. Medicine · 2024Article
- Regulating Lipid Metabolism in Gout: A New Perspective with Therapeutic Potential.International journal of general medicine · 2024Review
- HDL Functions-Current Status and Future Perspectives.Biomolecules · 2023Review
- Autoinflammatory Features in Gouty Arthritis.Journal of clinical medicine · 2021Review
- Native and oxidised lipoproteins negatively regulate the serum amyloid A-induced NLRP3 inflammasome activation in human macrophages.Clinical & translational immunology · 2021Article
- Autoinflammatory Mechanisms in Crystal-Induced Arthritis.Frontiers in medicine · 2020Review
- Curcumin attenuates MSU crystal-induced inflammation by inhibiting the degradation of IκBα and blocking mitochondrial damage.Arthritis research & therapy · 2019Article
- Better understanding of acute gouty attack using CT perfusion in a rabbit model.European radiology · 2019Article
- Phagocytosis of monosodium urate crystals by human synoviocytes induces inflammation.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- IL-33 Ameliorates the Development of MSU-Induced Inflammation Through Expanding MDSCs-Like Cells.Frontiers in endocrinology · 2019Article
- Study on the expressions of NLRP3 gene transcript variants in peripheral blood monocytes of primary gout patients.Clinical rheumatology · 2018Article
- High levels of oxidized fatty acids in HDL are associated with impaired HDL function in patients with active rheumatoid arthritis.Clinical rheumatology · 2018Article
- Disease-Associated Particulates and Joint Inflammation; Mechanistic Insights and Potential Therapeutic Targets.Frontiers in immunology · 2018Review
- The immunoglobulin D Fc receptor expressed on fibroblast-like synoviocytes from patients with rheumatoid arthritis contributes to the cell activation.Acta pharmacologica Sinica · 2017Article
- Hemin and Cobalt Protoporphyrin Inhibit NLRP3 Inflammasome Activation by Enhancing Autophagy: A Novel Mechanism of Inflammasome Regulation.Journal of innate immunity · 2017Article
- Ozonated autohemotherapy modulates the serum levels of inflammatory cytokines in gouty patients.Open access rheumatology : research and reviews · 2017Article
- How Factors Involved in the Resolution of Crystal-Induced Inflammation Target IL-1β.Frontiers in pharmacology · 2017Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionTo investigate whether monosodium urate (MSU) crystals induce the production of CCL2 (monocyte chemoattractant protein-1; MCP-1) in human fibroblast-like synoviocytes (FLS) and whether this mechanism would be affected by high-density lipoproteins (HDL).
methodsHuman FLS isolated from synovial tissue explants were stimulated with MSU crystals (0.01 to 0.5 mg/ml) or interleukin (IL)-1beta (10 pg/ml) in the presence or absence of HDL (50 and 100 microg/ml). The production and expression of CCL2 was evaluated with ELISA, confocal microscopy, immunofluorescence microscopy, chemotaxis assay, and real-time quantitative PCR.
resultsExposure of FLS to MSU crystals induced CCL2 accumulation in culture medium in a dose- and time-dependent manner, reaching a plateau at 50 to 75 microg/ml MSU crystals and 20 to 24 hours. Although low, the induced CCL2 levels were sufficient to trigger mononuclear cell migration. In resting FLS, CCL2 was localized in small cytoplasmic vesicles whose number diminished with MSU crystal stimulation. Concomitantly, MSU crystals triggered the induction of CCL2 mRNA expression. All these processes were inhibited by HDL, which cause a 50% decrease in CCL2 mRNA levels and a dose-dependent inhibition of the release of CCL2. Similar results were obtained when FLS were pretreated with HDL and washed before activation by MSU crystals or IL-1beta, suggesting a direct effect of HDL on the FLS activation state.
conclusionsThe present results demonstrate that MSU crystals induce FLS to release CCL2 that is stored in vesicles in resting conditions. This mechanism is inhibited by HDL, which may limit the inflammatory process by diminishing CCL2 production and, in turn, monocytes/macrophages recruitment in joints. This study confirms the antiinflammatory functions of HDL, which might play a part in the limitation of acute gout attack.
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