ArticleMolecular microbiology2010
A high-affinity putrescine-cadaverine transporter from Trypanosoma cruzi.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 66 citations in OpenAlex.
- Mechanistic Exploration ofPharmaceuticals (Basel, Switzerland) · 2026Article
- Quantitative assessment of the nanoanatomy of the contractile vacuole complex inLife science alliance · 2024Article
- Polyamine Metabolism for Drug Intervention in Trypanosomatids.Pathogens (Basel, Switzerland) · 2024Review
- Specific Gene Expression inGenes · 2023Article
- The old and the new about the contractile vacuole of Trypanosoma cruzi.The Journal of eukaryotic microbiology · 2022Review
- Polyamine Metabolism inMedical sciences (Basel, Switzerland) · 2022Review
- A CRISPR/Cas9-riboswitch-Based Method for Downregulation of Gene Expression inFrontiers in cellular and infection microbiology · 2020Article
- Identification ofFrontiers in medicine · 2019Article
- Polyamines in protozoan pathogens.The Journal of biological chemistry · 2018Review
- Ornithine uptake and the modulation of drug sensitivity inFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- The superfamily keeps growing: Identification in trypanosomatids of RibJ, the first riboflavin transporter family in protists.PLoS neglected tropical diseases · 2017Article
- Trypanosoma cruzi Polyamine Transporter: Its Role on Parasite Growth and Survival Under Stress Conditions.The Journal of membrane biology · 2016Article
- Discovery of novel polyamine analogs with anti-protozoal activity by computer guided drug repositioning.Journal of computer-aided molecular design · 2016Article
- A ComparativeEvolutionary bioinformatics online · 2016Article
- The Trypanosoma cruzi Diamine Transporter Is Essential for Robust Infection of Mammalian Cells.PloS one · 2016Article
- TcPho91 is a contractile vacuole phosphate sodium symporter that regulates phosphate and polyphosphate metabolism in Trypanosoma cruzi.Molecular microbiology · 2015Article
- Identification and functional characterization of a novel arginine/ornithine transporter, a member of a cationic amino acid transporter subfamily in the Trypanosoma cruzi genome.Parasites & vectors · 2015Article
- Rab32 is essential for maintaining functional acidocalcisomes, and for growth and infectivity of Trypanosoma cruzi.Journal of cell science · 2015Article
- Golgi UDP-GlcNAc:polypeptide O-α-N-Acetyl-d-glucosaminyltransferase 2 (TcOGNT2) regulates trypomastigote production and function in Trypanosoma cruzi.Eukaryotic cell · 2014Article
- Rab11 regulates trafficking of trans-sialidase to the plasma membrane through the contractile vacuole complex of Trypanosoma cruzi.PLoS pathogens · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Whereas mammalian cells and most other organisms can synthesize polyamines from basic amino acids, the protozoan parasite Trypanosoma cruzi is incapable of polyamine biosynthesis de novo and therefore obligatorily relies upon putrescine acquisition from the host to meet its nutritional requirements. The cell surface proteins that mediate polyamine transport into T. cruzi, as well as most eukaryotes, however, have by-in-large eluded discovery at the molecular level. Here we report the identification and functional characterization of two polyamine transporters, TcPOT1.1 and TcPOT1.2, encoded by alleles from two T. cruzi haplotypes. Overexpression of the TcPOT1.1 and TcPOT1.2 genes in T. cruzi epimastigotes revealed that TcPOT1.1 and TcPOT1.2 were high-affinity transporters that recognized both putrescine and cadaverine but not spermidine or spermine. Furthermore, the activities and subcellular locations of both TcPOT1.1 and TcPOT1.2 in intact parasites were profoundly influenced by extracellular putrescine availability. These results establish TcPOT1.1 and TcPOT1.2 as key components of the T. cruzi polyamine transport pathway, an indispensable nutritional function for the parasite that may be amenable to therapeutic manipulation.
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