ArticleProceedings of the National Academy of Sciences of the United States of America2010
Function and specificity of synthetic Hox transcription factors in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Review
- Generation of Hoxa11-3XFLAG and Hoxd11-3XFLAG alleles to investigate Hox11 genome-wide binding.Developmental biology · 2025Article
- Biology of Hox Genes: Questions and Technological Challenges.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Hox genes in development and beyond.Development (Cambridge, England) · 2023Article
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- Control of Hox transcription factor concentration and cell-to-cell variability by an auto-regulatory switch.Development (Cambridge, England) · 2019Article
- Drosophila melanogaster Hox transcription factors access the RNA polymerase II machinery through direct homeodomain binding to a conserved motif of mediator subunit Med19.PLoS genetics · 2014Article
- Developmental competence and the induction of ectopic proboscises in Drosophila melanogaster.Development genes and evolution · 2013Article
- Disentangling the many layers of eukaryotic transcriptional regulation.Annual review of genetics · 2012Review
- Variable motif utilization in homeotic selector (Hox)-cofactor complex formation controls specificity.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Functional synthetic Antennapedia genes and the dual roles of YPWM motif and linker size in transcriptional activation and repression.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Dissecting the functional specificities of two Hox proteins.Genes & development · 2010Article
- Quantitative study of synthetic Hox transcription factor-DNA interactions in live cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homeotic (Hox) genes encode transcription factors that confer segmental identity along the anteroposterior axis of the embryo. However the molecular mechanisms underlying Hox-mediated transcription and the differential requirements for specificity in the regulation of the vast number of Hox-target genes remain ill-defined. Here we show that synthetic Sex combs reduced (Scr) genes that encode the Scr C terminus containing the homedomain (HD) and YPWM motif (Scr-HD) are functional in vivo. Synthetic Scr-HD peptides can induce ectopic salivary glands in the embryo and homeotic transformations in the adult fly, act as transcriptional activators and repressors during development, and participate in protein-protein interactions. Their transformation capacity was found to be enhanced over their full-length counterpart and mutations known to transform the full-length protein into constitutively active or inactive variants behaved accordingly in the synthetic peptides. Our results show that synthetic Scr-HD genes are sufficient for homeotic function in Drosophila and suggest that the N terminus of Scr has a role in transcriptional potency, rather than specificity. We also demonstrate that synthetic peptides behave largely in a predictable way, by exhibiting Scr-specific phenotypes throughout development, which makes them an important tool for synthetic biology.
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