ArticleProceedings of the National Academy of Sciences of the United States of America2010
Quantitative study of synthetic Hox transcription factor-DNA interactions in live cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 64 citations in OpenAlex.
- Quantitative modulation of a spatial enhancer through the biophysical properties of a transcription factor binding site.Science advances · 2026Article
- Celebrating 50 years of fluorescence correlation spectroscopy (FCS): Advancing live-cell massively parallel FCS studies with photostable GFPs, mStayGold and StayGold/E138D.Biochimica et biophysica acta. General subjects · 2025Article
- Interaction of Receptor-Binding Domain of the SARS-CoV-2 Omicron Variant with hACE2 and Actin.Cells · 2024Article
- Long-range formation of the Bicoid gradient requires multiple dynamic modes that spatially vary across the embryo.Development (Cambridge, England) · 2024Article
- Non-Peptide Opioids Differ in Effects on Mu-Opioid (MOP) and Serotonin 1A (5-HTMolecules (Basel, Switzerland) · 2022Article
- Using FCS to accurately measure protein concentration in the presence of noise and photobleaching.Biophysical journal · 2021Article
- Article
- Capicua is a fast-acting transcriptional brake.Current biology : CB · 2021Article
- Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification.Journal of structural biology: X · 2021Article
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- Navigating the crowd: visualizing coordination between genome dynamics, structure, and transcription.Genome biology · 2020Review
- A strategy for designing allosteric modulators of transcription factor dimerization.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Yorkie controls tube length and apical barrier integrity during airway development.The Journal of cell biology · 2019Article
- Control of Hox transcription factor concentration and cell-to-cell variability by an auto-regulatory switch.Development (Cambridge, England) · 2019Article
- Relationship Between Homodimeric Glucocorticoid Receptor and Transcriptional Regulation Assessed via an In Vitro Fluorescence Correlation Spectroscopy-Microwell System.Scientific reports · 2018Article
- Interaction of the signaling state analog and the apoprotein form of the orange carotenoid protein with the fluorescence recovery protein.Photosynthesis research · 2018Article
- Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape.Scientific reports · 2017Article
- In vivo fluorescence correlation spectroscopy analyses of FMBP-1, a silkworm transcription factor.FEBS open bio · 2016Article
- Conformational analysis of misfolded protein aggregation by FRET and live-cell imaging techniques.International journal of molecular sciences · 2015Review
- Estimating binding properties of transcription factors from genome-wide binding profiles.Nucleic acids research · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription factor-DNA interactions are life sustaining and therefore the subject of intensive research. In spite of vast effort, quantitative in vivo studies of the molecular mechanisms underlying these fundamental interactions remain challenging. In the preceding paper, we designed synthetic Sex combs reduced (Scr) peptides and validated genetically their function as transcriptional regulators. Here we present a controllable system for quantitative studies of protein-DNA interactions in live cells that enables us to "titrate" the concentration of the synthetic Scr peptides in a single cell. Using methods with single-molecule sensitivity, advanced fluorescence imaging and fluorescence correlation spectroscopy (FCS), we were able to study the kinetics of Scr-DNA interactions in live salivary gland cells, where Scr is normally expressed during development. We discerned freely moving Scr molecules, characterized the specific and nonspecific Scr peptide-DNA interactions, and estimated their corresponding dissociation constants (K(d)) in vivo. Our results suggest that the synthetic Scr transcription factors find their specific target sites primarily by multiple association/dissociation events, the rapidity of which is largely owed to electrostatic interactions. Based on these new findings, we formulate a model mechanism and emulate the kinetics of Scr homeodomain-DNA interactions in live cells using numerical simulations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.