ArticleNucleic acids research2010
Slow non-specific accumulation of 2'-deoxy and 2'-O-methyl oligonucleotide probes at mitochondria in live cells.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- DNA Nanostructures for siRNA Delivery.Bioconjugate chemistry · 2026Review
- Dual-key cooperatively activated DNA regulator for controlling mitochondria-lysosome interactions.Nature communications · 2025Article
- Photocaged amplified FRET nanoflares: spatiotemporal controllable of mRNA-powered nanomachines for precise and sensitive microRNA imaging in live cells.Nucleic acids research · 2022Article
- Angiogenin promotes angiogenesis via the endonucleolytic decay of miR-141 in colorectal cancer.Molecular therapy. Nucleic acids · 2022Article
- Ratiometric Near-Infrared Fluorescent Probes Based on Hemicyanine Dyes Bearing Dithioacetal and Formal Residues for pH Detection in Mitochondria.Molecules (Basel, Switzerland) · 2021Article
- Chimeric Drug Design with a Noncharged Carrier for Mitochondrial Delivery.Pharmaceutics · 2021Article
- Adapting CRISPR/Cas9 System for Targeting Mitochondrial Genome.Frontiers in genetics · 2021Article
- DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids.Advances in experimental medicine and biology · 2021Article
- Nucleic Acid Hybrids as Advanced Antibacterial Nanocarriers.Pharmaceutics · 2020Review
- Anionic Polymers Promote Mitochondrial Targeting of Delocalized Lipophilic Cations.Bioconjugate chemistry · 2020Article
- Uptake and Fate of Fluorescently Labeled DNA Nanostructures in Cellular Environments: A Cautionary Tale.ACS central science · 2019Article
- Visualization of the Cellular Uptake and Trafficking of DNA Origami Nanostructures in Cancer Cells.Journal of the American Chemical Society · 2018Article
- hsa-miR-4485 regulates mitochondrial functions and inhibits the tumorigenicity of breast cancer cells.Journal of molecular medicine (Berlin, Germany) · 2017Article
- Pharmacokinetics on a microscale: visualizing Cy5-labeled oligonucleotide release from poly(n-butylcyanoacrylate) nanocapsules in cells.International journal of nanomedicine · 2014Article
- Tracking neuronal marker expression inside living differentiating cells using molecular beacons.Frontiers in cellular neuroscience · 2013Article
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Whole-body scanning PCR; a highly sensitive method to study the biodistribution of mRNAs, noncoding RNAs and therapeutic oligonucleotides.Nucleic acids research · 2013Article
- Sub-cellular temporal and spatial distribution of electrotransferred LNA/DNA oligomer.Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research · 2013Article
- Molecular beacons: powerful tools for imaging RNA in living cells.Journal of nucleic acids · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Molecular beacons (MBs) have the potential to provide a powerful tool for rapid RNA detection in living cells, as well as monitoring the dynamics of RNA expression in response to external stimuli. To exploit this potential, it is necessary to distinguish true signal from background signal due to non-specific interactions. Here, we show that, when cyanine-dye labeled 2'-deoxy and 2'-O-methyl oligonucleotide probes are inside living cells for >5 h, most of their signals co-localize with mitochondrial staining. These probes include random-sequence MB, dye-labeled single-strand linear oligonucleotide and dye-labeled double-stranded oligonucleotide. Using carbonyl cyanide m-chlorophenyl hydrazone treatment, we found that the non-specific accumulation of oligonucleotide probes at mitochondria was driven by mitochondrial membrane potential. We further demonstrated that the dye-labeled oligonucleotide probes were likely on/near the surface of mitochondria but not inside mitochondrial inner membrane. Interestingly, oligonucleotides probes labeled respectively with Alexa Fluor 488 and Alexa Fluor 546 did not accumulate at mitochondria, suggesting that the non-specific interaction between dye-labeled ODN probes and mitochondria is dye-specific. These results may help design and optimize fluorescence imaging probes for long-time RNA detection and monitoring in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.