Evidence map›Paper›PMID 20147412›Full record

ArticleJournal of virology2010

Hepatitis B virus replication induces methylation of both host and viral DNA.

Perumal Vivekanandan, Hubert Darius-J Daniel, Rajesh Kannangai, Francisco Martinez-Murillo, Michael Torbenson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed
4.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed, 141 citations in OpenAlex.

  1. Article
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  15. The role of HBV cccDNA in occult hepatitis B virus infection.Molecular and cellular biochemistry · 2023
    Review
  16. Article
  17. Review
  18. Review
  19. Review
  20. Article

14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Perumal VivekanandanDepartment of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.
Hubert Darius-J Daniel
Rajesh Kannangai
Francisco Martinez-Murillo
Michael Torbenson
Johns Hopkins University · US

Funding

Genetic and epigenetic regulation of Hepatitis B contributes to liver cancerR01DK078686 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI TORBENSON, MICHAEL S · 2008 to 2011
$1.4M
NIDDK NIH HHS R01 DK078686NIDDK NIH HHS R01DK078686
6 · The paper itself

Abstract

Control of viral replication is a major therapeutic goal to reduce morbidity and mortality from chronic hepatitis B virus (HBV) infection. Recently, methylation has been identified as a novel host defense mechanism, and methylation of viral DNA leads to downregulation of HBV gene expression. To better understand the mechanisms of HBV methylation, cell lines were exposed to HBV using a model system that mimics natural infection and the expression of host DNA methyltransferase genes (DNMTs) was measured. DNMT1, DNMT2, and DNMT3 were all significantly upregulated in response to HBV. DNMT3 was further studied because of its known role in the de novo methylation of DNA. Cotransfection experiments with full-length HBV and DNMT3 led to the downregulation of viral protein and pregenomic RNA production. To investigate whether the upregulation of DNMTs could also have an effect on the methylation of host DNA, cell lines were exposed to HBV in two independent model systems, one that mimics natural infection and a second model with temporary transfection. Host DNA methylation was measured by DNA microarray analysis. Increased methylation of host CpG islands was detected in both experimental systems. Two CpG islands, corresponding to genes SUFU and TIRAP, were selected, and the downregulation of these genes in hepatocellular carcinomas was confirmed. In conclusion, hepatocytes respond to HBV infection by upregulating DNMTs. The DNMTs methylate viral DNA, leading to decreased viral gene expression and decreased viral replication. However, virus-induced overexpression of DNMTs also leads to methylation of host CpG islands.

Indexed as

Virus ReplicationCell LineDNADNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3ADown-RegulationHepatitis B virusHepatocytesHumansLiverMethylationMicroarray AnalysisRNA, ViralUp-RegulationViral ProteinsDNADNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3ADNMT1 protein, humanDNMT3A protein, humanRNA, ViralTRDMT1 protein, humanViral Proteins

Identifiers

PMID20147412
PMCPMC2863779
OpenAlexW2098076096

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.