ArticleDevelopment (Cambridge, England)2010
Epithelial relaxation mediated by the myosin phosphatase regulator Mypt1 is required for brain ventricle lumen expansion and hindbrain morphogenesis.
Article in Development (Cambridge, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 89 citations in OpenAlex.
- Dynamic interactions between epithelial skin cells and a sensory cavity sculpt the growing olfactory orifice.Nature communications · 2026Article
- Model recapitulates regenerative limb blastema formation through local softening of the wounded epithelium.bioRxiv : the preprint server for biology · 2026Article
- A genetic model of congenital intestinal atresia implicates Mypt1 in epithelial organisation.Disease models & mechanisms · 2026Article
- myh9b is a critical non-muscle myosin II encoding gene that interacts with myh9a and myh10 during zebrafish development in both compensatory and redundant pathways.G3 (Bethesda, Md.) · 2025Article
- Sculpting with stiffness: rigidity as a regulator of morphogenesis.Biochemical Society transactions · 2023Review
- Hindbrain rhombomere centers harbor a heterogenous population of dividing progenitors which rely on Notch signaling.Frontiers in cell and developmental biology · 2023Article
- Morphogenetic Roles of Hydrostatic Pressure in Animal Development.Annual review of cell and developmental biology · 2022Review
- 16pdel lipid changes in iPSC-derived neurons and function of FAM57B in lipid metabolism and synaptogenesis.iScience · 2022Article
- Cellular mechanisms of reverse epithelial curvature in tissue morphogenesis.Frontiers in cell and developmental biology · 2022Review
- Intestinal and optic-cup organoids as tools for unveiling mechanics of self-organizing morphogenesis.Biophysics and physicobiology · 2022Article
- Generation of caudal-type serotonin neurons and hindbrain-fate organoids from hPSCs.Stem cell reports · 2021Article
- Segmentation and patterning of the vertebrate hindbrain.Development (Cambridge, England) · 2021Review
- Coordinated assembly and release of adhesions builds apical junctional belts duringDevelopment (Cambridge, England) · 2020Article
- The Evolution of Duplicated Genes of the Cpi-17/Phi-1 (International journal of molecular sciences · 2020Article
- Apical Constriction Reversal upon Mitotic Entry Underlies Different Morphogenetic Outcomes of Cell Division.Molecular biology of the cell · 2020Article
- Polyacrylamide Bead Sensors for in vivo Quantification of Cell-Scale Stress in Zebrafish Development.Scientific reports · 2019Article
- Actomyosin regulation by Eph receptor signaling couples boundary cell formation to border sharpness.eLife · 2019Article
- Role of Tricellular Tight Junction Protein Lipolysis-Stimulated Lipoprotein Receptor (LSR) in Cancer Cells.International journal of molecular sciences · 2019Review
- The extracellular matrix-myosin pathway in mechanotransduction: from molecule to tissue.Emerging topics in life sciences · 2018Article
- Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We demonstrate that in the zebrafish hindbrain, cell shape, rhombomere morphogenesis and, unexpectedly, brain ventricle lumen expansion depend on the contractile state of the neuroepithelium. The hindbrain neural tube opens in a specific sequence, with initial separation along the midline at rhombomere boundaries, subsequent openings within rhombomeres and eventual coalescence of openings into the hindbrain ventricle lumen. A mutation in the myosin phosphatase regulator mypt1 results in a small ventricle due to impaired stretching of the surrounding neuroepithelium. Although initial hindbrain opening remains normal, mypt1 mutant rhombomeres do not undergo normal morphological progression. Three-dimensional reconstruction demonstrates cell shapes within rhombomeres and at rhombomere boundaries are abnormal in mypt1 mutants. Wild-type cell shape requires that surrounding cells are also wild type, whereas mutant cell shape is autonomously regulated. Supporting the requirement for regulation of myosin function during hindbrain morphogenesis, wild-type embryos show dynamic levels of phosphorylated myosin regulatory light chain (pMRLC). By contrast, mutants show continuously high pMRLC levels, with concentration of pMRLC and myosin II at the apical side of the epithelium, and myosin II and actin concentration at rhombomere boundaries. Brain ventricle lumen expansion, rhombomere morphology and cell shape are rescued by inhibition of myosin II function, indicating that each defect is a consequence of overactive myosin. We suggest that the epithelium must ;relax', via activity of myosin phosphatase, to allow for normal hindbrain morphogenesis and expansion of the brain ventricular lumen. Epithelial relaxation might be a widespread strategy to facilitate tube inflation in many organs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.