ArticleHuman molecular genetics2010
Discrete subcellular partitioning of human retrotransposon RNAs despite a common mechanism of genome insertion.
Article in Human molecular genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Heritable L1 Retrotransposition Events During Development: Understanding Their Origins: Examination of heritable, endogenous L1 retrotransposition in mice opens up exciting new questions and research directions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2018Pooled it
- Review
- Primary Cell Culture as a Model System for Evolutionary Molecular Physiology.International journal of molecular sciences · 2024Review
- LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential.Nucleic acids research · 2024Article
- Dissolution of ribonucleoprotein condensates by the embryonic stem cell protein L1TD1.Nucleic acids research · 2024Article
- Co-expression of distinct L1 retrotransposon coiled coils can lead to their entanglement.Mobile DNA · 2023Article
- ZCCHC3 is a stress granule zinc knuckle protein that strongly suppresses LINE-1 retrotransposition.PLoS genetics · 2023Article
- Ribonucleoprotein Granules: Between Stress and Transposable Elements.Biomolecules · 2023Review
- Retrotransposon LINE-1 bodies in the cytoplasm of piRNA-deficient mouse spermatocytes: Ribonucleoproteins overcoming the integrated stress response.PLoS genetics · 2023Article
- Expression of L1 retrotransposons in granulocytes from patients with active systemic lupus erythematosus.Mobile DNA · 2023Article
- Generalized nuclear localization of retroelement transcripts.Mobile DNA · 2022Article
- The Role of Transposable Elements of the Human Genome in Neuronal Function and Pathology.International journal of molecular sciences · 2022Review
- MxB inhibits long interspersed element type 1 retrotransposition.PLoS genetics · 2022Article
- Hepatitis C virus infection restricts human LINE-1 retrotransposition in hepatoma cells.PLoS pathogens · 2021Article
- mRNA Targeting, Transport and Local Translation in Eukaryotic Cells: From the Classical View to a Diversity of New Concepts.Molecular biology · 2021Article
- C9orf72-associated SMCR8 protein binds in the ubiquitin pathway and with proteins linked with neurological disease.Acta neuropathologica communications · 2020Article
- Diverse transposable element landscapes in pathogenic and nonpathogenic yeast models: the value of a comparative perspective.Mobile DNA · 2020Review
- RNA ligation precedes the retrotransposition of U6/LINE-1 chimeric RNA.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Systematic Characterization of Stress-Induced RNA Granulation.Molecular cell · 2018Article
- Spliced integrated retrotransposed element (SpIRE) formation in the human genome.PLoS biology · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Despite the immense significance retrotransposons have had for genome evolution much about their biology is unknown, including the processes of forming their ribonucleoprotein (RNP) particles and transporting them about the cell. Suppression of retrotransposon expression, together with the presence of retrotransposon sequence within numerous mRNAs, makes tracking endogenous L1 RNP particles in cells problematic. We overcame these difficulties by assaying in living and fixed cells tagged-RNPs generated from constructs expressing retrotransposition-competent L1s. In this way, we demonstrate for the first time the subcellular colocalization of L1 RNA and proteins ORF1p and ORF2p, and show their targeting together to cytoplasmic foci. Foci are often associated with markers of cytoplasmic stress granules. Furthermore, mutation analyses reveal that ORF1p can direct L1 RNP distribution within the cell. We also assayed RNA localization of the non-autonomous retrotransposons Alu and SVA. Despite a requirement for the L1 integration machinery, each manifests unique features of subcellular RNA distribution. In nuclei Alu RNA forms small round foci partially associated with marker proteins for coiled bodies, suborganelles involved in the processing of non-coding RNAs. SVA RNA patterning is distinctive, being cytoplasmic but without prominent foci and concentrated in large nuclear aggregates that often ring nucleoli. Such variability predicts significant differences in the life cycles of these elements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.