ArticleThe Journal of biological chemistry2010
Targeting acetylcholinesterase to membrane rafts: a function mediated by the proline-rich membrane anchor (PRiMA) in neurons.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- The Tacrine-Induced Endoplasmic Reticulum Stress in AChE-Expressed Cells Leads to Improper Assembly and Transport of the Oligomeric Enzyme: Reversal by Trehalose.Journal of neurochemistry · 2025Article
- Microarray-Based Methodology for Lipid Profiling, Enzymatic Activity, And Binding Assays in Printed Lipid Raft Membranes from Astrocytes and Neurons.Analytical chemistry · 2025Article
- A Structured Brain-wide and Genome-wide Association Study Using ADNI PET Images.The Canadian journal of statistics = Revue canadienne de statistique · 2021Article
- Interacting withActa pharmaceutica Sinica. B · 2020Article
- Non-neuronal Role of Acetylcholinesterase in Bone Development and Degeneration.Frontiers in cell and developmental biology · 2020Review
- Biochemical adaptation in brain Acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish Tilapia mossambica.Scientific reports · 2019Article
- Alzheimer's Disease as a Membrane Disorder: Spatial Cross-Talk Among Beta-Amyloid Peptides, Nicotinic Acetylcholine Receptors and Lipid Rafts.Frontiers in cellular neuroscience · 2019Review
- Jia-Wei-Kai-Xin-San, an Herbal Medicine Formula, Ameliorates Cognitive Deficits via Modulating Metabolism of Beta Amyloid Protein and Neurotrophic Factors in Hippocampus of AβFrontiers in pharmacology · 2019Article
- Genistein, a Phytoestrogen in Soybean, Induces the Expression of Acetylcholinesterase via G Protein-Coupled Receptor 30 in PC12 Cells.Frontiers in molecular neuroscience · 2018Article
- Colorectal adenoma and cancer detection based on altered methylation pattern of SFRP1, SFRP2, SDC2, and PRIMA1 in plasma samples.Epigenetics · 2017Article
- Wnt3a induces the expression of acetylcholinesterase during osteoblast differentiation via the Runx2 transcription factor.The Journal of biological chemistry · 2017Article
- Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality Disorder.International journal of molecular sciences · 2016Article
- Three N-Glycosylation Sites of Human Acetylcholinesterase Shares Similar Glycan Composition.Journal of molecular neuroscience : MN · 2015Article
- DNA hypermethylation and decreased mRNA expression of MAL, PRIMA1, PTGDR and SFRP1 in colorectal adenoma and cancer.BMC cancer · 2015Article
- Interaction of prion protein with acetylcholinesterase: potential pathobiological implications in prion diseases.Acta neuropathologica communications · 2015Article
- Hypercholesterolemia induces short-term spatial memory impairments in mice: up-regulation of acetylcholinesterase activity as an early and causal event?Journal of neural transmission (Vienna, Austria : 1996) · 2014Article
- Translocator protein/peripheral benzodiazepine receptor is not required for steroid hormone biosynthesis.Endocrinology · 2014Article
- Popeye domain containing 1 (Popdc1/Bves) is a caveolae-associated protein involved in ischemia tolerance.PloS one · 2013Article
- The transmembrane region is responsible for targeting of adaptor protein LAX into "heavy rafts".PloS one · 2012Article
- Lipid rafts and Alzheimer's disease: protein-lipid interactions and perturbation of signaling.Frontiers in physiology · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the mammalian brain, acetylcholinesterase (AChE) is anchored in cell membranes by a transmembrane protein PRiMA (proline-rich membrane anchor). We present evidence that at least part of the PRiMA-linked AChE is integrated in membrane microdomains called rafts. A significant proportion of PRiMA-linked AChE tetramers from rat brain was recovered in raft fractions; this proportion was markedly higher at low rather than at high concentrations of cold Triton X-100. The detergent-resistant fraction increased during brain development. In NG108-15 neuroblastoma cells transfected with cDNAs encoding AChE(T) and PRiMA, PRiMA-linked G(4) AChE was found in membrane rafts and showed the same sensitivity to cold Triton X-100 extraction as in the brain. The association of PRiMA-linked AChE with rafts was weaker than that of glycosylphosphatidylinositol-anchored G(2) AChE or G(4) Q(N)-H(C)-linked AChE. It was found to depend on the presence of a cholesterol-binding motif, called CRAC (cholesterol recognition/interaction amino acid consensus), located at the junction of transmembrane and cytoplasmic domains of both PRiMA I and II isoforms. The cytoplasmic domain of PRiMA, which differs between PRiMA I and PRiMA II, appeared to play some role in stabilizing the raft localization of G(4) AChE, because the Triton X-100-resistant fraction was smaller with the shorter PRiMA II isoform than that with the longer PRiMA I isoform.
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Registered trials
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