ArticleBMC microbiology2010
Development of a toxR-based loop-mediated isothermal amplification assay for detecting Vibrio parahaemolyticus.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 106 citations in OpenAlex.
- Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia.Frontiers in microbiology · 2026Article
- Colorimetric and electrochemical analysis of DNAzyme-LAMP amplicons for the detection of Escherichia coli in food matrices.Scientific reports · 2024Article
- Vibrio parahaemolyticus Epidemiology and Pathogenesis: Novel Insights on an Emerging Foodborne Pathogen.Advances in experimental medicine and biology · 2023Article
- Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental water.BMC microbiology · 2022Article
- Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples.Scientific reports · 2022Article
- Point-of-care system for rapid real-time detection of SARS-CoV-2 virus based on commercially available Arduino platforms.Frontiers in bioengineering and biotechnology · 2022Article
- Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research.Animals : an open access journal from MDPI · 2021Review
- Qualitative and Quantitative Detection of Potentially VirulentPathogens (Basel, Switzerland) · 2021Article
- Distribution of phenotypic and genotypic antimicrobial resistance and virulence genes in Vibrio parahaemolyticus isolated from cultivated oysters and estuarine water.FEMS microbiology ecology · 2020Article
- Isolation, Molecular Characterization and Antibiotic Susceptibility Pattern ofJournal of microbiology and biotechnology · 2020Article
- Rapid visual detection of Vibrio parahaemolyticus in seafood samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye.World journal of microbiology & biotechnology · 2020Article
- Development and evaluation of a rapid and sensitive RPA assay for specific detection of Vibrio parahaemolyticus in seafood.BMC microbiology · 2019Article
- Classification of Multiple DNA Dyes Based on Inhibition Effects on Real-Time Loop-Mediated Isothermal Amplification (LAMP): Prospect for Point of Care Setting.Frontiers in microbiology · 2019Article
- A Comparative Study of Three Detection Techniques forBioMed research international · 2018Article
- Amplicon Competition Enables End-Point Quantitation of Nucleic Acids Following Isothermal Amplification.Chembiochem : a European journal of chemical biology · 2017Article
- Development of a multiplex loop-mediated isothermal amplification method for the simultaneous detection of Salmonella spp. and Vibrio parahaemolyticus.Scientific reports · 2017Article
- Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in <25min.Journal of microbiological methods · 2017Article
- A primerless molecular diagnostic: phosphorothioated-terminal hairpin formation and self-priming extension (PS-THSP).Analytical and bioanalytical chemistry · 2016Article
- Rapid and Sensitive Detection of Vibrio parahaemolyticus and Vibrio vulnificus by Multiple Endonuclease Restriction Real-Time Loop-Mediated Isothermal Amplification Technique.Molecules (Basel, Switzerland) · 2016Article
- An Innovative Method for Rapid Identification and Detection of Vibrio alginolyticus in Different Infection Models.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVibrio parahaemolyticus is a leading cause of seafood-related bacterial gastroenteritis and outbreaks worldwide. Sensitive and specific detection methods are needed to better control V. parahaemolyticus infections. This study aimed at developing a highly specific and sensitive loop-mediated isothermal amplification (LAMP) assay for detecting V. parahaemolyticus in oysters. A set of five LAMP primers, two outer, two inner, and one loop were designed based on the published V. parahaemolyticus toxR sequence. Specificity of the assay was evaluated using a panel of 36 V. parahaemolyticus and 39 other strains. The assay sensitivity was determined using serial dilutions of V. parahaemolyticus ATCC 27969 culture ranging from 10(8) CFU/ml to extinction. The assay was also tested in experimentally inoculated oyster samples.
resultsThe toxR-based LAMP assay was able to specifically detect all of the 36 V. parahaemolyticus strains without amplification from 39 other strains. The detection limit was 47-470 cells per reaction in pure culture, up to 100-fold more sensitive than that of toxR-PCR. When applied in spiked oysters, the assay was able to detect 1.1 x 10(5) V. parahaemolyticus cells per gram of oyster without enrichment, up to 100-fold more sensitive than that of toxR-PCR. Standard curves generated for detecting V. parahaemolyticus in both pure culture and spiked oyster samples showed good linear relationship between cell numbers and the fluorescence or turbidity signals.
conclusionsThe toxR-based LAMP assay developed in this study was sensitive, specific, and quantitative, holding great potential for future field detection of V. parahaemolyticus in raw oysters.
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Registered trials
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