ArticleJournal of visualized experiments : JoVE2010
Preparation of Drosophila polytene chromosome squashes for antibody labeling.
Article in Journal of visualized experiments : JoVE, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- ILP4 and InR regulate paclitaxel-induced hypersensitivity differently inMolecular biology of the cell · 2026Article
- Maternally inherited siRNAs initiate piRNA cluster formation.Molecular cell · 2023Article
- Heat-induced SIRT1-mediated H4K16ac deacetylation impairs resection and SMARCAD1 recruitment to double strand breaks.iScience · 2022Article
- Preparation ofBio-protocol · 2020Article
- JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila.Nature communications · 2019Article
- Article
- Differential Occupancy of Two GA-Binding Proteins Promotes Targeting of the Drosophila Dosage Compensation Complex to the Male X Chromosome.Cell reports · 2018Article
- Article
- H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation.Development (Cambridge, England) · 2017Article
- The essential Drosophila CLAMP protein differentially regulates non-coding roX RNAs in male and females.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2017Article
- Direct interrogation of the role of H3K9 in metazoan heterochromatin function.Genes & development · 2016Article
- Quantitative Measurement of Histone Tail Acetylation Reveals Stage-Specific Regulation and Response to Environmental Changes during Drosophila Development.Biochemistry · 2016Article
- Digitor/dASCIZ Has Multiple Roles in Drosophila Development.PloS one · 2016Article
- Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes.Nature communications · 2015Article
- Article
- Genome-wide analysis of regulation of gene expression and H3K9me2 distribution by JIL-1 kinase mediated histone H3S10 phosphorylation in Drosophila.Nucleic acids research · 2014Article
- Domain requirements of the JIL-1 tandem kinase for histone H3 serine 10 phosphorylation and chromatin remodeling in vivo.The Journal of biological chemistry · 2013Article
- Evidence against a role for the JIL-1 kinase in H3S28 phosphorylation and 14-3-3 recruitment to active genes in Drosophila.PloS one · 2013Article
- High-throughput physical mapping of chromosomes using automated in situ hybridization.Journal of visualized experiments : JoVE · 2012Article
- The chromodomain-containing NH(2)-terminus of Chromator interacts with histone H1 and is required for correct targeting to chromatin.Chromosoma · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Drosophila has long been a favorite model system for studying the relationship between chromatin structure and gene regulation due to the cytological advantages provided by the giant salivary gland polytene chromosomes of third instar larvae. In this tissue the chromosomes undergo many rounds of replication in the absence of cell division giving rise to approximately 1000 copies. The DNA remains aligned after each replicative cycle resulting in greatly enlarged chromosomes that provide a unique opportunity to correlate chromatin morphology with the localization of specific proteins. Consequently, there has been a high level of interest in defining the epigenetic modifications present at different genes and at different stages of the transcription process. An important tool for such studies is the labeling of polytene chromosomes with antibodies to the enzyme, transcription factor, or histone modification of interest. This video protocol illustrates the squash technique used in the Johansen laboratory to prepare Drosophila polytene chromosomes for antibody labeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.