ArticleNucleic acids research2010
Strong dependence between functional domains in a dual-function snoRNA infers coupling of rRNA processing and modification events.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Passing the anaerobic threshold is associated with substantial changes in the gene expression profile in white blood cells.European journal of applied physiology · 2012Trial
- Ribosomes: from conserved origin to functional/medical mobility and heterogeneity.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- The plant epitranscriptome: revisiting pseudouridine and 2'-O-methyl RNA modifications.Plant biotechnology journal · 2022Review
- RNA folding and functions of RNA helicases in ribosome biogenesis.RNA biology · 2022Review
- Emerging Functions for snoRNAs and snoRNA-Derived Fragments.International journal of molecular sciences · 2021Review
- The multistructural forms of box C/D ribonucleoprotein particles.RNA (New York, N.Y.) · 2018Review
- Stepwise and dynamic assembly of the earliest precursors of small ribosomal subunits in yeast.Genes & development · 2016Article
- DEAD-box RNA helicase Dbp4 is required for small-subunit processome formation and function.Molecular and cellular biology · 2015Article
- A pre-ribosomal RNA interaction network involving snoRNAs and the Rok1 helicase.RNA (New York, N.Y.) · 2014Article
- Rrp5 binding at multiple sites coordinates pre-rRNA processing and assembly.Molecular cell · 2013Article
- Ribosome biogenesis in the yeast Saccharomyces cerevisiae.Genetics · 2013Review
- An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme.PLoS biology · 2013Article
- Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA.Nucleic acids research · 2013Article
- Are snoRNAs and snoRNA host genes new players in cancer?Nature reviews. Cancer · 2012Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Most small nucleolar RNAs (snoRNAs) guide rRNA nucleotide modifications, some participate in pre-rRNA cleavages, and a few have both functions. These activities involve direct base-pairing of the snoRNA with pre-rRNA using different domains. It is not known if the modification and processing functions occur independently or in a coordinated manner. We address this question by mutational analysis of a yeast box H/ACA snoRNA that mediates both processing and modification. This snoRNA (snR10) contains canonical 5'- and 3'-hairpin structures with a guide domain for pseudouridylation in the 3' hairpin. Our functional mapping results show that: (i) processing requires the 5' hairpin exclusively, in particular a 7-nt element; (ii) loss of the 3' hairpin or pseudouridine does not affect rRNA processing; (iii) a single nucleotide insertion in the guide domain shifts modification to an adjacent uridine in rRNA, and severely impairs both processing and cell growth; and (iv) the deleterious effects of the insertion mutation depend on the presence of the processing element in the 5' hairpin, but not modification of the novel site. Together, the results suggest that the snoRNA hairpins function in a coordinated manner and that their interactions with pre-rRNA could be coupled.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.