ArticleNucleic acids research2010
Rapid interactome profiling by massive sequencing.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 104 citations in OpenAlex.
- Combining Yeast Display and Bacterial Genomic Library for the Unbiased Isolation of Novel Polysaccharide-Binding Peptides.International journal of molecular sciences · 2026Article
- Hepatokines: unveiling the molecular and cellular mechanisms connecting hepatic tissue to insulin resistance and inflammation.Acta diabetologica · 2024Review
- Review
- Bacteriophages as Potential Tools for Use in Antimicrobial Therapy and Vaccine Development.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Mapping Protein-Protein Interaction Interface Peptides with Jun-Fos Assisted Phage Display and Deep Sequencing.ACS synthetic biology · 2020Article
- InteractomeSeq: a web server for the identification and profiling of domains and epitopes from phage display and next generation sequencing data.Nucleic acids research · 2020Article
- The RNA-binding protein ILF3 binds to transposable element sequences in SINEUP lncRNAs.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- High-resolution protein-protein interaction mapping using all-The Journal of biological chemistry · 2019Article
- Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing.Journal of visualized experiments : JoVE · 2018Article
- Phage display peptide libraries: deviations from randomness and correctives.Nucleic acids research · 2018Article
- Screening the Molecular Framework Underlying Local Dendritic mRNA Translation.Frontiers in molecular neuroscience · 2017Review
- Mining gut microbiome oligopeptides by functional metaproteome display.Scientific reports · 2016Article
- Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display.Frontiers in microbiology · 2016Review
- High-throughput methods for identification of protein-protein interactions involving short linear motifs.Cell communication and signaling : CCS · 2015Article
- High-throughput sequencing enhanced phage display enables the identification of patient-specific epitope motifs in serum.Scientific reports · 2015Article
- FASTAptamer: A Bioinformatic Toolkit for High-throughput Sequence Analysis of Combinatorial Selections.Molecular therapy. Nucleic acids · 2015Article
- Identification of novel proteins binding the AU-rich element of α-prothymosin mRNA through the selection of open reading frames (RIDome).RNA biology · 2015Article
- From deep sequencing to actual clones.Protein engineering, design & selection : PEDS · 2014Article
- An Air-Well sparging minifermenter system for high-throughput protein production.Microbial cell factories · 2014Article
- Interaction analysis through proteomic phage display.BioMed research international · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a high-throughput protein expression and interaction analysis platform that combines cDNA phage display library selection and massive gene sequencing using the 454 platform. A phage display library of open reading frame (ORF) fragments was created from mRNA derived from different tissues. This was used to study the interaction network of the enzyme transglutaminase 2 (TG2), a multifunctional enzyme involved in the regulation of cell growth, differentiation and apoptosis, associated with many different pathologies. After two rounds of panning with TG2 we assayed the frequency of ORFs within the selected phage population using 454 sequencing. Ranking and analysis of more than 120,000 sequences allowed us to identify several potential interactors, which were subsequently confirmed in functional assays. Within the identified clones, three had been previously described as interacting proteins (fibronectin, SMOC1 and GSTO2), while all the others were new. When compared with standard systems, such as microtiter enzyme-linked immunosorbant assay, the method described here is dramatically faster and yields far more information about the interaction under study, allowing better characterization of complex systems. For example, in the case of fibronectin, it was possible to identify the specific domains involved in the interaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.