ArticleMatrix biology : journal of the International Society for Matrix Biology2010
Tissue section AFM: In situ ultrastructural imaging of native biomolecules.
Article in Matrix biology : journal of the International Society for Matrix Biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 114 citations in OpenAlex.
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- The mechanical properties measurement could be affected by forceps: a technical note on sampling human annulus fibrosus.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2024Article
- A method for analyzing AFM force mapping data obtained from soft tissue cryosections.Journal of biomechanics · 2024Article
- Atomic force microscopy for characterization of decellularized extracellular matrix (dECM) based materials.Science and technology of advanced materials · 2024Review
- Morphological changes in the anterior vaginal wall caused by aging: a scanning electron microscopy study.International urogynecology journal · 2023Article
- Micro-mechanical fingerprints of the rat bladder change in actinic cystitis and tumor presence.Communications biology · 2023Article
- Aggrecan governs intervertebral discs development by providing critical mechanical cues of the extracellular matrix.Frontiers in bioengineering and biotechnology · 2023Article
- Nanomechanical and Morphological AFM Mapping of Normal Tissues and Tumors on Live Brain Slices Using Specially Designed Embedding Matrix and Laser-Shaped Cantilevers.Biomedicines · 2022Article
- Atomic Force Microscopy (AFM) Applications in Arrhythmogenic Cardiomyopathy.International journal of molecular sciences · 2022Review
- Assessing Collagen D-Band Periodicity with Atomic Force Microscopy.Materials (Basel, Switzerland) · 2022Review
- X-ray microtomography reveals a lattice-like network within aortic elastic lamellae.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Late Changes in the Extracellular Matrix of the Bladder after Radiation Therapy for Pelvic Tumors.Diagnostics (Basel, Switzerland) · 2021Article
- Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy.Scientific reports · 2021Article
- HDAC Inhibition Reverses Preexisting Diastolic Dysfunction and Blocks Covert Extracellular Matrix Remodeling.Circulation · 2021Article
- Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
Conventional approaches for ultrastructural high-resolution imaging of biological specimens induce profound changes in bio-molecular structures. By combining tissue cryo-sectioning with non-destructive atomic force microscopy (AFM) imaging we have developed a methodology that may be applied by the non-specialist to both preserve and visualize bio-molecular structures (in particular extracellular matrix assemblies) in situ. This tissue section AFM technique is capable of: i) resolving nm-microm scale features of intra- and extracellular structures in tissue cryo-sections; ii) imaging the same tissue region before and after experimental interventions; iii) combining ultrastructural imaging with complimentary microscopical and micromechanical methods. Here, we employ this technique to: i) visualize the macro-molecular structures of unstained and unfixed fibrillar collagens (in skin, cartilage and intervertebral disc), elastic fibres (in aorta and lung), desmosomes (in nasal epithelium) and mitochondria (in heart); ii) quantify the ultrastructural effects of sequential collagenase digestion on a single elastic fibre; iii) correlate optical (auto fluorescent) with ultrastructural (AFM) images of aortic elastic lamellae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.