ArticleTissue engineering. Part C, Methods2010
Effects of hyperinsulinemia on lipolytic function of three-dimensional adipocyte/endothelial co-cultures.
Article in Tissue engineering. Part C, Methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Adipose Tissue Engineering Biomaterials: Smart Scaffolds, Vascularization, and Clinical Frontiers.Biomolecules · 2026Review
- Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases.Theranostics · 2024Review
- Development of a More Environmentally Friendly Silk Fibroin Scaffold for Soft Tissue Applications.Journal of functional biomaterials · 2023Article
- Clinical outcomes of drug-coated balloon for treatment of de novo coronary artery disease with and without diabetes.Saudi medical journal · 2022Article
- 3D bioprinted white adipose model forBiofabrication · 2022Article
- Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix.Biomimetics (Basel, Switzerland) · 2021Article
- Impact of Hyperinsulinemia on Long-Term Clinical Outcomes of Percutaneous Coronary Intervention in Patients without Diabetes Who Have Acute Myocardial Syndrome.Diabetes, metabolic syndrome and obesity : targets and therapy · 2021Article
- Fattening chips: hypertrophy, feeding, and fasting of human white adipocytesLab on a chip · 2020Article
- Mechanisms of action, chemical characteristics, and model systems of obesogens.BMC biomedical engineering · 2020Review
- Human Adipose Derived Cells in Two- and Three-Dimensional Cultures: Functional Validation of an In Vitro Fat Construct.Stem cells international · 2020Article
- Article
- Elucidating the role of plexin D1 in body fat distribution and susceptibility to metabolic disease using a zebrafish model system.Adipocyte · 2017Article
- Development of a Three-Dimensional Adipose Tissue Model for Studying Embryonic Exposures to Obesogenic Chemicals.Annals of biomedical engineering · 2017Article
- The Use of Silk as a Scaffold for Mature, Sustainable Unilocular Adipose 3D Tissue Engineered Systems.Advanced healthcare materials · 2016Article
- Opportunities and challenges in three-dimensional brown adipogenesis of stem cells.Biotechnology advances · 2015Review
- Long term perfusion system supporting adipogenesis.Methods (San Diego, Calif.) · 2015Article
- In vitro 3D full-thickness skin-equivalent tissue model using silk and collagen biomaterials.Macromolecular bioscience · 2012Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The increased incidence of both type 2 diabetes mellitus and obesity has prompted the need to develop physiologically relevant adipose tissue models for controlled study of both normal and diseased adipose functions. Insulin resistance, characteristic of both type 2 diabetes mellitus and obesity, is often preceded by hyperinsulinemia. We propose here a three-dimensional (3D) co-culture adipose tissue model to study the effects of high insulin exposure, which enabled the study of physiological cell responses to hyperinsulinemic conditions. Two-dimensional adipocyte studies were initially conducted to establish a baseline control in which insulin levels were established. Adipocytes and endothelial cells were subsequently co-cultured on 3D porous silk fibroin scaffolds in normal or high insulin concentrations, and their physiological responses were assessed with respect to lipogenesis and lipolysis. High insulin levels stimulated both an increase in triglyceride accumulation and a decrease in lipolysis levels compared to that of normal insulin conditions. In contrast, adipocyte monocultures did not exhibit any differences between insulin levels. The ability of this 3D system to elicit physiological responses to hyperinsulinemia in co-culture serves as a significant step forward in adipose tissue engineering. The development of physiologically relevant 3D in vitro adipose tissue models presents promise for the study of disease mechanisms as well as in assessing therapeutic treatments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.