ArticleStem cells and development2010
Subfractionation of differentiating human embryonic stem cell populations allows the isolation of a mesodermal population enriched for intermediate mesoderm and putative renal progenitors.
Article in Stem cells and development, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Challenges and outcomes in pediatric and adult kidney transplantation.Frontiers in pediatrics · 2026Review
- Human reconstructed kidney models.In vitro cellular & developmental biology. Animal · 2021Review
- Regenerative Medicine, Disease Modeling, and Drug Discovery in Human Pluripotent Stem Cell-derived Kidney Tissue.European Medical Journal. Reproductive health · 2017Article
- Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells.Scientific reports · 2017Article
- Kidney Organoids: A Translational Journey.Trends in molecular medicine · 2017Review
- Does Renal Repair Recapitulate Kidney Development?Journal of the American Society of Nephrology : JASN · 2017Review
- Generation of kidney tubular organoids from human pluripotent stem cells.Scientific reports · 2016Article
- A strategy for generating kidney organoids: Recapitulating the development in human pluripotent stem cells.Developmental biology · 2016Review
- Making new kidneys: On the road from science fiction to science fact.Current opinion in organ transplantation · 2016Review
- Understanding kidney morphogenesis to guide renal tissue regeneration.Nature reviews. Nephrology · 2016Review
- Direct reprogramming of human bone marrow stromal cells into functional renal cells using cell-free extracts.Stem cell reports · 2015Article
- Renal progenitors derived from human iPSCs engraft and restore function in a mouse model of acute kidney injury.Scientific reports · 2015Article
- Directed Differentiation of Pluripotent Stem Cells into Kidney.Biomarker insights · 2015Review
- Disease modeling using human induced pluripotent stem cells: lessons from the liver.Biochimica et biophysica acta · 2015Review
- Bioengineering kidneys for transplantation.Seminars in nephrology · 2014Review
- Directed differentiation of pluripotent stem cells to kidney cells.Seminars in nephrology · 2014Review
- Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers.Journal of the American Society of Nephrology : JASN · 2014Article
- Recreating kidney progenitors from pluripotent cells.Pediatric nephrology (Berlin, Germany) · 2014Review
- Stem cells: potential and challenges for kidney repair.American journal of physiology. Renal physiology · 2014Review
- New opportunities: harnessing induced pluripotency for discovery in diabetes and metabolism.Cell metabolism · 2013Review
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
Abstract
Human embryonic stem (ES) cells are pluripotent and are believed to be able to generate all cell types in the body. As such, they have potential applications in regenerative therapy for kidney disease. However, before this can be achieved, a protocol to differentiate human ES cells to mesodermal renal progenitor lineages is required. Reduction of serum concentration and feeder layer density reduction cultures were used to differentiate human ES cells for 14 days. Differentiated ES cells were then fractionated by flow cytometry based on expression of the markers CD24, podocalyxin, and GCTM2 to isolate putative renal cells. These cells up-regulated the expression of the renal transcription factors PAX2, LHX1, and WT1 when compared with unfractionated human ES cells. Immunohistochemical assays confirmed that a subset of cells within this fraction co-expressed nuclear WT1 and PAX2 proteins. Transcriptome profiling also showed that the most differentially up-regulated genes in this fraction preferentially associated with kidney development in comparison with any other lineage. When compared with a transcriptome profile database of urogenital development (GUDMAP), the top 200 differentially up-regulated genes in this fraction strongly clustered into a group of genes associated with the metanephric mesenchyme at E11.5 and the corticonephrogenic interstitium at E15.5 of murine kidney development. Hence, this approach confirms an ability to direct human ES cells toward a renal progenitor state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.