ArticleParasitology research2010
The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri.
Article in Parasitology research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 52 citations in OpenAlex.
- Identification of Naegleria fowleri antigens recognized by serum antibodies from people of Mexicali Valley, México.Parasitology research · 2025Article
- Eating the brain - A multidisciplinary study provides new insights into the mechanisms underlying the cytopathogenicity of Naegleria fowleri.PLoS pathogens · 2025Article
- Cloning of nf-profilin and intercellular interaction with nf-actin in Naegleria fowleri cysts.Scientific reports · 2025Article
- Understanding the pathogenicity of Naegleria fowleri in association with N. fowleri antigen-1 (Nfa1).Parasites, hosts and diseases · 2024Review
- Therapeutic glycan-specific antibody binding mediates protection during primary amoebic meningoencephalitis.Infection and immunity · 2024Article
- ArePathogens (Basel, Switzerland) · 2024Review
- Amoebicidal effect of chlorine dioxide gas against pathogenic Naegleria fowleri and Acanthamoeba polyphaga.Parasitology research · 2024Article
- Transcriptomic profiling of "brain-eating amoeba"Frontiers in cellular and infection microbiology · 2024Article
- De Novo Transcriptome Profiling ofPathogens (Basel, Switzerland) · 2023Article
- Characterization of the extracellular vesicles, ultrastructural morphology, and intercellular interactions of multiple clinical isolates of the brain-eating amoeba,Frontiers in microbiology · 2023Article
- Comparative transcriptome profiling of virulent and avirulent isolates of Neoparamoeba perurans.Scientific reports · 2022Article
- Article
- Comparative Genomic and Transcriptomic Analysis of Naegleria fowleri Clinical and Environmental Isolates.mSphere · 2021Article
- Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri.BMC biology · 2021Article
- Host-Parasite Interaction of Atlantic salmon (Frontiers in immunology · 2021Article
- The Multiple Roles of Trogocytosis in Immunity, the Nervous System, and Development.BioMed research international · 2021Review
- Conserved actin machinery drives microtubule-independent motility and phagocytosis in Naegleria.The Journal of cell biology · 2020Article
- Identification of Immunogenic Antigens ofPathogens (Basel, Switzerland) · 2020Article
- Fowlerstefin, a cysteine protease inhibitor of Naegleria fowleri, induces inflammatory responses in BV-2 microglial cells in vitro.Parasites & vectors · 2020Article
- Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Naegleria fowleri destroys target cells by trogocytosis, a phagocytosis mechanism, and a process of piecemeal ingestion of target cells by food-cups. Phagocytosis is an actin-dependent process that involves polymerization of monomeric G-actin into filamentous F-actin. However, despite the numerous studies concerning phagocytosis, its role in the N. fowleri food-cup formation related with trogocytosis has been poorly reported. In this study, we cloned and characterized an Nf-actin gene to elucidate the role of Nf-actin gene in N. fowleri pathogenesis. The Nf-actin gene is composed of 1,128-bp and produced a 54.1-kDa recombinant protein (Nf-actin). The sequence identity was 82% with nonpathogenic Naegleria gruberi but has no sequence identity with other mammals or human actin gene. Anti-Nf-actin polyclonal antibody was produced in BALB/c mice immunized with recombinant Nf-actin. The Nf-actin was localized on the cytoplasm, pseudopodia, and especially, food-cup structure (amoebastome) in N. fowleri trophozoites using immunofluorescence assay. When N. fowleri co-cultured with Chinese hamster ovary cells, Nf-actin was observed to localize around on phagocytic food-cups. We also observed that N. fowleri treated with cytochalasin D as actin polymerization inhibitor or transfected with antisense oligomer of Nf-actin gene had shown the reduced ability of food-cup formation and in vitro cytotoxicity. Finally, it suggests that Nf-actin plays an important role in phagocytic activity of pathogenic N. fowleri.
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