ArticleProceedings of the National Academy of Sciences of the United States of America1991
Sequence of a cysteine-rich galactose-specific lectin of Entamoeba histolytica.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 141 citations in OpenAlex.
- Phylogenetic analyses of Chilomastix and Retortamonas species using in vitro excysted flagellates.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2023Article
- Evaluation of the PEJournal of immunology research · 2021Article
- Endoplasmic reticulum-resident Rab8A GTPase is involved in phagocytosis in the protozoan parasite Entamoeba histolytica.Cellular microbiology · 2016Article
- Entamoeba histolytica: adhesins and lectins in the trophozoite surface.Molecules (Basel, Switzerland) · 2015Review
- Expression, purification, and evaluation of recombinant LecA as a candidate for an amebic colitis vaccine.Vaccine · 2014Article
- The antiretroviral lectin cyanovirin-N targets well-known and novel targets on the surface of Entamoeba histolytica trophozoites.Eukaryotic cell · 2010Article
- The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic.PLoS neglected tropical diseases · 2010Article
- Localization and targeting of an unusual pyridine nucleotide transhydrogenase in Entamoeba histolytica.Eukaryotic cell · 2010Article
- Evidence for a "wattle and daub" model of the cyst wall of entamoeba.PLoS pathogens · 2009Article
- Evidence of a continuous endoplasmic reticulum in the protozoan parasite Entamoeba histolytica.Eukaryotic cell · 2008Article
- Endoplasmic reticulum continuity in the protozoan parasite Entamoeba histolytica: Evolutionary implications and a cautionary note.Communicative & integrative biology · 2008Article
- Transcriptional gene silencing reveals two distinct groups of Entamoeba histolytica Gal/GalNAc-lectin light subunits.Eukaryotic cell · 2007Article
- Unique posttranslational modifications of chitin-binding lectins of Entamoeba invadens cyst walls.Eukaryotic cell · 2006Article
- Identification and gene expression analysis of a large family of transmembrane kinases related to the Gal/GalNAc lectin in Entamoeba histolytica.Eukaryotic cell · 2005Article
- Roles of cell adhesion and cytoskeleton activity in Entamoeba histolytica pathogenesis: a delicate balance.Infection and immunity · 2005Article
- Amoebae as training grounds for intracellular bacterial pathogens.Applied and environmental microbiology · 2005Review
- Entamoeba histolytica lectins contain unique 6-Cys or 8-Cys chitin-binding domains.Infection and immunity · 2002Article
- Diagnosis of amebic liver abscess and intestinal infection with the TechLab Entamoeba histolytica II antigen detection and antibody tests.Journal of clinical microbiology · 2000Article
- The most abundant glycoprotein of amebic cyst walls (Jacob) is a lectin with five Cys-rich, chitin-binding domains.Infection and immunity · 2000Article
- Pathogenesis of intestinal amebiasis: from molecules to disease.Clinical microbiology reviews · 2000Review
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Entamoeba histolytica trophozoites adhere to human colonic mucins and epithelial cells by a cell surface galactose-specific lectin. This lectin, which is composed of two subunits linked by disulfide bonds, has been shown to be a protective antigen in an animal model of amebiasis. We have determined the sequence of the mature form of the 170-kDa heavy subunit from cDNA clones and PCR-amplified fragments. The heavy subunit sequence consisted of a putative extracellular domain containing 1209 amino acids with 16 potential sites for N-linked glycosylation, a 26-amino acid hydrophobic region, and a 41-amino acid cytoplasmic tail. The presence of N-linked oligosaccharides was confirmed by culturing amebae with tunicamycin, which resulted in a decrease in the heavy subunit molecular mass to 160 kDa and a loss of lectin activity. The extracellular domain was remarkable for an extensive cysteine-rich domain that shared identify with similar regions of several other cell surface proteins and appeared to confer protease resistance to the subunit.
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