ArticleProceedings of the National Academy of Sciences of the United States of America2010
Uridylation of mature miRNAs and siRNAs by the MUT68 nucleotidyltransferase promotes their degradation in Chlamydomonas.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 141 citations in OpenAlex.
- RNA Tailing by Nucleotidyltransferases in Plants: Mechanisms, Functions, and Biological Significance.Plants (Basel, Switzerland) · 2026Review
- Target-Directed miRNA Degradation: Mechanisms and Significance.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Unveiling the wheat NTP gene family: insights from genome-wide identification and TaNTP6 haplotype analysis.BMC plant biology · 2025Article
- Global identification of AGO3-RNA interactions reveals targets of small RNA-mediated gene regulation in Chlamydomonas reinhardtii.Plant & cell physiology · 2025Article
- Review
- Poly(U) polymerase activity in Caenorhabditis elegans regulates abundance and tailing of sRNA and mRNA.Genetics · 2024Article
- Identification and Expression Analysis of the Nucleotidyl Transferase Protein (NTP) Family in Soybean (International journal of molecular sciences · 2024Article
- microRNAs in action: biogenesis, function and regulation.Nature reviews. Genetics · 2023Review
- Small RNAs >26 nt in length associate with AGO1 and are upregulated by nutrient deprivation in the alga Chlamydomonas.The Plant cell · 2023Article
- Angiogenin promotes angiogenesis via the endonucleolytic decay of miR-141 in colorectal cancer.Molecular therapy. Nucleic acids · 2022Article
- Up-to-date on the evidence linking miRNA-related epitranscriptomic modifications and disease settings. Can these modifications affect cross-kingdom regulation?RNA biology · 2021Article
- Review
- Structural basis for guide RNA trimming by RNase D ribonuclease in Trypanosoma brucei.Nucleic acids research · 2021Article
- Identification of oligo-adenylated small RNAs in the parasite Entamoeba and a potential role for small RNA control.BMC genomics · 2020Article
- CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA.BMC biology · 2020Article
- Degradation of MicroRNA miR-466d-3p by Japanese Encephalitis Virus NS3 Facilitates Viral Replication and Interleukin-1β Expression.Journal of virology · 2020Article
- An ortholog of the Vasa intronic gene is required for small RNA-mediated translation repression inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Global analyses of the dynamics of mammalian microRNA metabolism.Genome research · 2019Article
- The RNase PARN Controls the Levels of Specific miRNAs that Contribute to p53 Regulation.Molecular cell · 2019Article
- Structural insights into a unique preference for 3' terminal guanine of mirtron in Drosophila TUTase tailor.Nucleic acids research · 2019Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulation of gene expression by small RNAs ( approximately 20-30 nucleotides in length) plays an essential role in developmental pathways and defense responses against genomic parasites in eukaryotes. MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) commonly direct the inactivation of cognate sequences through a variety of mechanisms, including RNA degradation, translation inhibition, and transcriptional repression. Recent studies have provided considerable insight into the biogenesis and the mode of action of miRNAs and siRNAs. However, relatively little is known about mechanisms of quality control and small RNA decay in RNA interference (RNAi) pathways. Here we show that deletion of MUT68, encoding a terminal nucleotidyltransferase in the alga Chlamydomonas reinhardtii, results in elevated miRNA and siRNA levels. We found that MUT68 plays a role in the untemplated uridylation of the 3' ends of small RNAs in vivo and stimulates their degradation by the RRP6 exosome subunit in vitro. Moreover, RRP6 depletion also leads to accumulation of small RNAs in vivo. We propose that MUT68 and RRP6 cooperate in the degradation of mature miRNAs and siRNAs, as a quality control mechanism to eliminate dysfunctional or damaged small RNA molecules.
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