ArticleBiophysical journal2010
Quantitative determination of spatial protein-protein correlations in fluorescence confocal microscopy.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
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- Aplp1 interacts with Lag3 to facilitate transmission of pathologic α-synuclein.Nature communications · 2024Article
- Imaging-based study demonstrates how the DEK nanoscale distribution differentially correlates with epigenetic marks in a breast cancer model.Scientific reports · 2023Article
- Glutathione Contribution in Interactions betweenInternational journal of molecular sciences · 2023Article
- Glucagon-like Peptide-1 Receptor in the Human Hypothalamus Is Associated with Body Mass Index and Colocalizes with the Anorexigenic Neuropeptide Nucleobindin-2/Nesfatin-1.International journal of molecular sciences · 2022Article
- Super-resolution imaging uncovers the nanoscopic segregation of polarity proteins in epithelia.eLife · 2022Article
- AtGSTU19 and AtGSTU24 as Moderators of the Response ofInternational journal of molecular sciences · 2022Article
- Glutathione Modulation in PVYInternational journal of molecular sciences · 2022Article
- Modulation of Expression of PVYVaccines · 2021Article
- Nanoscale Distribution of Nuclear Sites by Super-Resolved Image Cross-Correlation Spectroscopy.Biophysical journal · 2019Article
- Secretion of Phospholipase Dδ Functions as a Regulatory Mechanism in Plant Innate Immunity.The Plant cell · 2019Article
- MitoBKThe Journal of physiology · 2019Article
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- Fluorescence lifetime imaging nanoscopy for measuring Förster resonance energy transfer in cellular nanodomains.Neurophotonics · 2019Article
- Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics.Nature communications · 2018Article
- Article
- MatCol: a tool to measure fluorescence signal colocalisation in biological systems.Scientific reports · 2017Article
- Chronic hypoxia attenuates the vasodilator efficacy of protein kinase G in fetal and adult ovine cerebral arteries.American journal of physiology. Heart and circulatory physiology · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
To quantify spatial protein-protein proximity (colocalization) in paired microscopic images of two sets of proteins labeled by distinct fluorophores, we showed that the cross-correlation and the autocorrelation functions of image intensity consisted of fast and slowly decaying components. The fast component resulted from clusters of proteins specifically labeled, and the slow component resulted from image heterogeneity and a broadly-distributed background. To better evaluate spatial proximity between the two specifically labeled proteins, we extracted the fast-decaying component by fitting the sharp peak in correlation functions to a Gaussian function, which was then used to obtain protein-protein proximity index and the Pearson's correlation coefficient. We also employed the median-filter method as a universal approach for background reduction to minimize nonspecific fluorescence. We illustrated our method by analyzing computer-simulated images and biological images.
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