ArticleNucleic acids research1991
Promoters with the octamer DNA motif (ATGCAAAT) can be ubiquitous or cell type-specific depending on binding affinity of the octamer site and Oct-factor concentration.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 80 citations in OpenAlex.
- HSV-1 selectively packs the transcription factor Oct-1 into EVs to facilitate its infection.Frontiers in microbiology · 2023Article
- Oct4 mediates Müller glia reprogramming and cell cycle exit during retina regeneration in zebrafish.Life science alliance · 2019Article
- CTCF binding site sequence differences are associated with unique regulatory and functional trends during embryonic stem cell differentiation.Nucleic acids research · 2014Article
- Octamer-binding transcription factors: genomics and functions.Frontiers in bioscience (Landmark edition) · 2013Review
- The solution structure of ZNF593 from Homo sapiens reveals a zinc finger in a predominantly unstructured protein.Protein science : a publication of the Protein Society · 2008Article
- Activated Fes protein tyrosine kinase induces terminal macrophage differentiation of myeloid progenitors (U937 cells) and activation of the transcription factor PU.1.Molecular and cellular biology · 2002Article
- The Drosophila homolog of mammalian zinc finger factor MTF-1 activates transcription in response to heavy metals.Molecular and cellular biology · 2001Article
- Target gene search for the metal-responsive transcription factor MTF-1.Nucleic acids research · 2001Article
- Characterization of the mouse gene for the heavy metal-responsive transcription factor MTF-1.Cell stress & chaperones · 2000Article
- Using altered specificity Oct-1 and Oct-2 mutants to analyze the regulation of immunoglobulin gene transcription.The EMBO journal · 1997Article
- Concerted activity of host cell factor subregions in promoting stable VP16 complex assembly and preventing interference by the acidic activation domain.Molecular and cellular biology · 1997Article
- Cellular factors controlling the activity of woodchuck hepatitis virus enhancer II.Journal of virology · 1996Article
- The B cell coactivator Bob1 shows DNA sequence-dependent complex formation with Oct-1/Oct-2 factors, leading to differential promoter activation.The EMBO journal · 1996Article
- Transcriptional activation by Oct-3: evidence for a specific role of the POU-specific domain in mediating functional interaction with Oct-1.Nucleic acids research · 1996Article
- Transcription factors interacting with herpes simplex virus alpha gene promoters in sensory neurons.Nucleic acids research · 1995Article
- GATA-binding proteins regulate the human gonadotropin alpha-subunit gene in the placenta and pituitary gland.Molecular and cellular biology · 1994Article
- Differential transactivation potential of Oct1 and Oct2 is determined by additional B cell-specific activities.The EMBO journal · 1994Article
- Analysis of the imperfect octamer-containing human immunoglobulin VH6 gene promoter.Nucleic acids research · 1994Article
- Stably integrated mouse mammary tumor virus long terminal repeat DNA requires the octamer motifs for basal promoter activity.Molecular and cellular biology · 1994Article
- Multiple motifs regulate the B-cell-specific promoter of the B29 gene.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunoglobulin (Ig) gene promoters contain the octamer sequence motif ATGCAAAT which is recognized by cellular transcription factors (Oct factors). Besides the ubiquitous Oct-1 factor, there is also a group of related factors (Oct-2 factors) encoded by a separate gene. The Oct-2 gene is regulated in a cell-type specific manner, and the protein is present in large amounts in B lymphocytes. We have previously shown that simple composite promoters of an octamer/TATA box type are poorly active in non-B cells but are strongly responsive to ectopic expression of Oct-2A factor, a major representative of the lymphocyte Oct-2 factors. In the present study we have tested the activity of a number of composite promoters and natural Ig promoters, and their response to Oct-1 and Oct-2 factors. Unexpectedly, we find that octamer/TATA promoters with a high affinity octamer site direct ubiquitous expression. By contrast, promoter constructions that behave in a B cell-specific manner tend to have a weak octamer binding site. These promoters are responsive to ectopic expression of additional Oct-factor, irrespective of whether it is Oct-1 or Oct-2. Using natural Ig promoters rather than composite promoters, we find that an IgH promoter is well transcribed in non-B cells via the ubiquitous Oct-1 factor, while Ig kappa and Ig lambda light chain promoters require additional Oct factor for maximal expression. It seems therefore likely that during B cell differentiation, Ig heavy chain promoters can be activated by Oct-1, before the appearance of Oct-2 factors. Oct-2 factors then would serve to boost the expression from Ig light chain promoters, which are known to be activated only after successful heavy chain gene rearrangement.
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