ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2010
Low-cost, high-throughput, automated counting of bacterial colonies.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed.
- Safety and Immunogenicity of a Candidate Bioconjugate Vaccine against Shigella flexneri 2a Administered to Healthy Adults: a Single-Blind, Randomized Phase I Study.Clinical and vaccine immunology : CVI · 2016Trial
- Engineering Compact Base Editors by AlphaFold-Guided Mutation Scan and Escherichia coli-Based Tri-Selection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Colony-YOLO: A Lightweight Micro-Colony Detection Network Based on Improved YOLOv8n.Microorganisms · 2025Article
- Antibacterial compounds against non-growing and intracellular bacteria.npj antimicrobials and resistance · 2025Article
- Integrated and high-throughput method to collect, store, recover, and manage microbial isolates in mini-arrays.Microbiology spectrum · 2025Article
- Article
- Real-time imaging of bacterial colony growth dynamics for cells with Type IV-A1 CRISPR-Cas activity.microLife · 2025Article
- UMicroorganisms · 2024Article
- High-Content and High-Throughput Clonogenic Survival Assay Using Fluorescence Barcoding.Cancers · 2023Article
- Evaluation of an Automated System for the Counting of Microbial Colonies.Microbiology spectrum · 2023Article
- A review of open-source image analysis tools for mammalian cell culture: algorithms, features and implementations.PeerJ. Computer science · 2023Article
- Article
- Machine learning for enumeration of cell colony forming units.Visual computing for industry, biomedicine, and art · 2022Article
- Methods to monitor bacterial growth and replicative rates at the single-cell level.FEMS microbiology reviews · 2022Review
- Immunization of Rabbits with a QuadrivalentmSphere · 2022Article
- Image Analysis Semi-Automatic System for Colony-Forming-Unit Counting.Bioengineering (Basel, Switzerland) · 2022Article
- Dihydroconiferyl Ferulate Isolated fromPharmaceuticals (Basel, Switzerland) · 2022Article
- A New Few-Shot Learning Method of Bacterial Colony Counting Based on the Edge Computing Device.Biology · 2022Article
- A comprehensive review of image analysis methods for microorganism counting: from classical image processing to deep learning approaches.Artificial intelligence review · 2022Article
- Scan4CFU: Low-cost, open-source bacterial colony tracking over large areas and extended incubation times.HardwareX · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Research involving bacterial pathogens often requires enumeration of bacteria colonies. Here, we present a low-cost, high-throughput colony counting system consisting of colony counting software and a consumer-grade digital camera or document scanner. We demonstrate that this software, called "NICE" (NIST's Integrated Colony Enumerator), can count bacterial colonies as part of a high-throughput multiplexed opsonophagocytic killing assay used to characterize pneumococcal vaccine efficacy. The results obtained with NICE correlate well with the results obtained from manual counting, with a mean difference of less than 3%. NICE is also rapid; it can count colonies from multiple reaction wells within minutes and export the results to a spreadsheet for data processing. As this program is freely available from NIST, NICE should be helpful in bacteria colony enumeration required in many microbiological studies, and in standardizing colony counting methods.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.