ArticlePLoS pathogens2010
Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors.
Article in PLoS pathogens, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 113 citations in OpenAlex.
- Attenuation mechanisms and vaccine potential of the serial passage-derived Pasteurella multocida strain PMZ8 in ducks.Poultry science · 2026Article
- Cepharanthine as a potential Fis inhibitor enhances Enrofloxacin efficacy against Pasteurella multocida.BMC microbiology · 2026Article
- Comparative genomics andFrontiers in microbiology · 2026Article
- Comparative analysis of shared and unique mechanisms important for diverse strains of Pasteurella multocida to cause systemic infection in mice.PLoS pathogens · 2025Article
- Deleting fis downregulates virulence and effectively protects Pasteurella multocida infection in mice.BMC veterinary research · 2025Article
- Characterization of a Capsule-DeficientMicroorganisms · 2025Article
- Hemorrhagic septicemia: A major threat to livestock health.Open veterinary journal · 2025Review
- Attenuated vaccine PmCQ2Δ4555-4580 effectively protects mice against Pasteurella multocida infection.BMC veterinary research · 2024Article
- Genome-Wide Investigation of Pasteurella multocida Identifies the Stringent Response as a Negative Regulator of Hyaluronic Acid Capsule Production.Microbiology spectrum · 2022Article
- The Role and Targets of the RNA-Binding Protein ProQ in the Gram-Negative Bacterial Pathogen Pasteurella multocida.Journal of bacteriology · 2022Article
- Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks.Veterinary research · 2022Article
- Virulence Determinants and Antimicrobial Profiles ofAntibiotics (Basel, Switzerland) · 2021Article
- The lipopolysaccharide outer core transferase genes pcgD and hptE contribute differently to the virulence of Pasteurella multocida in ducks.Veterinary research · 2021Article
- Fis Connects Two Sensory Pathways, Quorum Sensing and Surface Sensing, to Control Motility inFrontiers in microbiology · 2021Article
- Article
- Identification of Fur inFrontiers in veterinary science · 2019Article
- Emergence of High-Level Colistin Resistance in an Acinetobacter baumannii Clinical Isolate Mediated by Inactivation of the Global Regulator H-NS.Antimicrobial agents and chemotherapy · 2018Article
- Determination of the small RNA GcvB regulon in the Gram-negative bacterial pathogenRNA (New York, N.Y.) · 2018Article
- Article
- Characterization of Two Novel Lipopolysaccharide Phosphoethanolamine Transferases in Pasteurella multocida and Their Role in Resistance to Cathelicidin-2.Infection and immunity · 2017Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
P. multocida is the causative agent of a wide range of diseases of animals, including fowl cholera in poultry and wild birds. Fowl cholera isolates of P. multocida generally express a capsular polysaccharide composed of hyaluronic acid. There have been reports of spontaneous capsule loss in P. multocida, but the mechanism by which this occurs has not been determined. In this study, we identified three independent strains that had spontaneously lost the ability to produce capsular polysaccharide. Quantitative RT-PCR showed that these strains had significantly reduced transcription of the capsule biosynthetic genes, but DNA sequence analysis identified no mutations within the capsule biosynthetic locus. However, whole-genome sequencing of paired capsulated and acapsular strains identified a single point mutation within the fis gene in the acapsular strain. Sequencing of fis from two independently derived spontaneous acapsular strains showed that each contained a mutation within fis. Complementation of these strains with an intact copy of fis, predicted to encode a transcriptional regulator, returned capsule expression to all strains. Therefore, expression of a functional Fis protein is essential for capsule expression in P. multocida. DNA microarray analysis of one of the spontaneous fis mutants identified approximately 30 genes as down-regulated in the mutant, including pfhB_2, which encodes a filamentous hemagglutinin, a known P. multocida virulence factor, and plpE, which encodes the cross protective surface antigen PlpE. Therefore these experiments define for the first time a mechanism for spontaneous capsule loss in P. multocida and identify Fis as a critical regulator of capsule expression. Furthermore, Fis is involved in the regulation of a range of other P. multocida genes including important virulence factors.
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