ArticlePloS one2010
Staphylococcal PknB as the first prokaryotic representative of the proline-directed kinases.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 50 citations in OpenAlex.
- Phosphorylation by the PknB Serine/Threonine Kinase Stimulates Dimer Formation by WalR.Biochemistry · 2026Article
- Functional and Proteomic Dissection of the Contributions of CodY, SigB and the Hibernation Promoting Factor HPF to Interactions ofJournal of proteome research · 2024Article
- High-throughput functional analysis provides novel insight into type VII secretion inOpen biology · 2024Article
- A membrane-depolarizing toxin substrate of theProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Metabolic conversion of CI-1040 turns a cellular MEK-inhibitor into an antibacterial compound.Scientific reports · 2018Article
- EssC is a specificity determinant for Staphylococcus aureus type VII secretion.Microbiology (Reading, England) · 2018Article
- Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390.Microbiology (Reading, England) · 2017Article
- Functional analysis of the EsaB component of theMicrobiology (Reading, England) · 2017Article
- Article
- The type VII secretion system of Staphylococcus aureus secretes a nuclease toxin that targets competitor bacteria.Nature microbiology · 2016Article
- Proteomic discovery of host kinase signaling in bacterial infections.Proteomics. Clinical applications · 2016Review
- Membrane interactions and self-association of components of the Ess/Type VII secretion system of Staphylococcus aureus.FEBS letters · 2016Article
- The current state of the art of quantitative phosphoproteomics and its applications to diabetes research.Expert review of proteomics · 2016Review
- In Staphylococcus aureus the regulation of pyruvate kinase activity by serine/threonine protein kinase favors biofilm formation.3 Biotech · 2015Article
- Elucidating Host-Pathogen Interactions Based on Post-Translational Modifications Using Proteomics Approaches.Frontiers in microbiology · 2015Review
- Heterogeneity in ess transcriptional organization and variable contribution of the Ess/Type VII protein secretion system to virulence across closely related Staphylocccus aureus strains.Molecular microbiology · 2014Article
- Bacterial serine/threonine protein kinases in host-pathogen interactions.The Journal of biological chemistry · 2014Review
- Regulation of transcription by eukaryotic-like serine-threonine kinases and phosphatases in Gram-positive bacterial pathogens.Virulence · 2014Review
- A serine/threonine phosphatase encoded by MG_207 of Mycoplasma genitalium is critical for its virulence.BMC microbiology · 2013Article
- Pneumococcal gene complex involved in resistance to extracellular oxidative stress.Infection and immunity · 2012Article
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eukaryotic cell types, virtually all cellular processes are under control of proline-directed kinases and especially MAP kinases. Serine/threonine kinases in general were originally considered as a eukaryote-specific enzyme family. However, recent studies have revealed that orthologues of eukaryotic serine/threonine kinases exist in bacteria. Moreover, various pathogenic species, such as Yersinia and Mycobacterium, require serine/threonine kinases for successful invasion of human host cells. The substrates targeted by bacterial serine/threonine kinases have remained largely unknown. Here we report that the serine/threonine kinase PknB from the important pathogen Staphylococcus aureus is released into the external milieu, which opens up the possibility that PknB does not only phosphorylate bacterial proteins but also proteins of the human host. To identify possible human targets of purified PknB, we studied in vitro phosphorylation of peptide microarrays and detected 68 possible human targets for phosphorylation. These results show that PknB is a proline-directed kinase with MAP kinase-like enzymatic activity. As the potential cellular targets for PknB are involved in apoptosis, immune responses, transport, and metabolism, PknB secretion may help the bacterium to evade intracellular killing and facilitate its growth. In apparent agreement with this notion, phosphorylation of the host-cell response coordinating transcription factor ATF-2 by PknB was confirmed by mass spectrometry. Taken together, our results identify PknB as the first prokaryotic representative of the proline-directed kinase/MAP kinase family of enzymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.