ArticleNature methods2010
Silencing neurotransmission with membrane-tethered toxins.
Article in Nature methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 53 citations in OpenAlex.
- A genetically encoded secreted toxin potentiates synaptic NMDA receptors in hippocampal neurons and confers neuroprotection.PNAS nexus · 2025Article
- Design of Ultrapotent Genetically Encoded Inhibitors of Kv4.2 for Gating Neural Plasticity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- A high-affinity cocaine binding site associated with the brain acid soluble protein 1.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Direct activation of the proton channel by albumin leads to human sperm capacitation and sustained release of inflammatory mediators by neutrophils.Nature communications · 2021Article
- Neuronal genes deregulated in Cornelia de Lange Syndrome respond to removal and re-expression of cohesin.Nature communications · 2021Article
- Tethered peptide neurotoxins display two blocking mechanisms in the KScience advances · 2020Article
- ASTN2 modulates synaptic strength by trafficking and degradation of surface proteins.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Retrograde inhibition by a specific subset of interpeduncular α5 nicotinic neurons regulates nicotine preference.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Review
- Article
- Cocaine-Induced Synaptic Alterations in Thalamus to Nucleus Accumbens Projection.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2016Article
- Excitatory transmission at thalamo-striatal synapses mediates susceptibility to social stress.Nature neuroscience · 2015Article
- Suppression of Peripheral Pain by Blockade of Voltage-Gated Calcium 2.2 Channels in Nociceptors Induces RANKL and Impairs Recovery From Inflammatory Arthritis in a Mouse Model.Arthritis & rheumatology (Hoboken, N.J.) · 2015Article
- Ion channel engineering: perspectives and strategies.Journal of molecular biology · 2015Review
- Article
- Habenular expression of rare missense variants of the β4 nicotinic receptor subunit alters nicotine consumption.Frontiers in human neuroscience · 2014Article
- A fully genetically encoded protein architecture for optical control of peptide ligand concentration.Nature communications · 2014Article
- Pharmacological Inhibition of Voltage-gated Ca(2+) Channels for Chronic Pain Relief.Current neuropharmacology · 2013Article
- Membrane-tethered ligands: tools for cell-autonomous pharmacological manipulation of biological circuits.Physiology (Bethesda, Md.) · 2013Review
- Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
At synaptic terminals, high voltage-activated Ca(v)2.1 and Ca(v)2.2 calcium channels have an essential and joint role in coupling the presynaptic action potential to neurotransmitter release. Here we show that membrane-tethered toxins allowed cell-autonomous blockade of each channel individually or simultaneously in mouse neurons in vivo. We report optimized constitutive, inducible and Cre recombinase-dependent lentiviral vectors encoding fluorescent recombinant toxins, and we also validated the toxin-based strategy in a transgenic mouse model. Toxins delivered by lentiviral vectors selectively inhibited the dopaminergic nigrostriatal pathway, and transgenic mice with targeted expression in nociceptive peripheral neurons displayed long-lasting suppression of chronic pain. Optimized tethered toxins are tools for cell-specific and temporal manipulation of ion channel-mediated activities in vivo, including blockade of neurotransmitter release.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.