ArticleThe Journal of biological chemistry2010
The XBP-Bax1 helicase-nuclease complex unwinds and cleaves DNA: implications for eukaryal and archaeal nucleotide excision repair.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Sulfolobales: Acidothermophilic archaea as models for biology and biotechnological applications.Engineering microbiology · 2026Review
- Integrating Structural, Biochemical, and Cellular Perspectives on the TFIIH Helicases XPB and XPD.Biomolecules · 2026Review
- COL4A1, negatively regulated by XPD and miR-29a-3p, promotes cell proliferation, migration, invasion and epithelial-mesenchymal transition in liver cancer cells.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2021Article
- Structural basis of the XPB helicase-Bax1 nuclease complex interacting with the repair bubble DNA.Nucleic acids research · 2020Article
- Archaeal DNA Repair Mechanisms.Biomolecules · 2020Review
- Structural basis of the XPB-Bax1 complex as a dynamic helicase-nuclease machinery for DNA repair.Nucleic acids research · 2020Article
- Article
- Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15A.Extremophiles : life under extreme conditions · 2016Article
- Host nucleotide polymorphism in hepatitis B virus-associated hepatocellular carcinoma.World journal of hepatology · 2016Review
- Nanobiomotors of archaeal DNA repair machineries: current research status and application potential.Cell & bioscience · 2014Review
- Trypanosoma brucei harbours a divergent XPB helicase paralogue that is specialized in nucleotide excision repair and conserved among kinetoplastid organisms.Molecular microbiology · 2013Article
- Enzymatic activities and DNA substrate specificity of Mycobacterium tuberculosis DNA helicase XPB.PloS one · 2012Article
- Development of a simvastatin selection marker for a hyperthermophilic acidophile, Sulfolobus islandicus.Applied and environmental microbiology · 2012Article
- Defense islands in bacterial and archaeal genomes and prediction of novel defense systems.Journal of bacteriology · 2011Article
- Review
- Single-stranded DNA binding activity of XPBI, but not XPBII, from Sulfolobus tokodaii causes double-stranded DNA melting.Extremophiles : life under extreme conditions · 2011Article
- Repair of DNA double-strand breaks following UV damage in three Sulfolobus solfataricus strains.Journal of bacteriology · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
XPB helicase is an integral part of transcription factor TFIIH, required for both transcription initiation and nucleotide excision repair (NER). Along with the XPD helicase, XPB plays a crucial but only partly understood role in defining and extending the DNA repair bubble around lesions in NER. Archaea encode clear homologues of XPB and XPD, and structural studies of these proteins have yielded key insights relevant to the eukaryal system. Here we show that archaeal XPB functions with a structure-specific nuclease, Bax1, as a helicase-nuclease machine that unwinds and cleaves model NER substrates. DNA bubbles are extended by XPB and cleaved by Bax1 at a position equivalent to that cut by the XPG nuclease in eukaryal NER. The helicase activity of archaeal XPB is dependent on the conserved Thumb domain, which may act as the helix breaker. The N-terminal damage recognition domain of XPB is shown to be crucial for XPB-Bax1 activity and may be unique to the archaea. These findings have implications for the role of XPB in both archaeal and eukaryal NER and for the evolution of the NER pathway. XPB is shown to be a very limited helicase that can act on small DNA bubbles and open a defined region of the DNA duplex. The specialized functions of the accessory domains of XPB are now more clearly delineated. This is also the first direct demonstration of a repair function for archaeal XPB and suggests strongly that the role of XPB in transcription occurred later in evolution than that in repair.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.