ArticleArteriosclerosis, thrombosis, and vascular biology2010
Magnetic resonance molecular imaging of thrombosis in an arachidonic acid mouse model using an activated platelet targeted probe.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Molecular-gated MRIMaterials today. Bio · 2026Article
- Advanced targeted nanomedicines for vulnerable atherosclerosis plaque imaging and their potential clinical implications.Frontiers in pharmacology · 2022Review
- Platelets in Multiple Sclerosis: Early and Central Mediators of Inflammation and Neurodegeneration and Attractive Targets for Molecular Imaging and Site-Directed Therapy.Frontiers in immunology · 2021Review
- An unmet clinical need: The history of thrombus imaging.Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology · 2019Review
- Molecular Imaging of the Heart.Comprehensive Physiology · 2019Review
- Peptide-based fibrin-targeting probes for thrombus imaging.Dalton transactions (Cambridge, England : 2003) · 2017Review
- FeInternational journal of nanomedicine · 2017Article
- Direct Thrombus Imaging in Stroke.Journal of stroke · 2016Review
- Advances in MRI for the evaluation of carotid atherosclerosis.The British journal of radiology · 2015Review
- Design of superparamagnetic nanoparticles for magnetic particle imaging (MPI).International journal of molecular sciences · 2013Article
- MR and Targeted Molecular MRI of Vulnerable Plaques.Interventional neurology · 2013Review
- Preparation and characterization of gadolinium-loaded PLGA particles surface modified with RGDS for the detection of thrombus.International journal of nanomedicine · 2013Article
- Advances in molecular imaging of atherosclerosis and myocardial infarction: shedding new light on in vivo cardiovascular biology.American journal of physiology. Heart and circulatory physiology · 2012Review
- Strategies for Target-Specific Contrast Agents for Magnetic Resonance Imaging.Current molecular imaging · 2012Article
- Detection of thrombus size and protein content by ex vivo magnetization transfer and diffusion weighted MRI.Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance · 2012Article
- Article
- Diagnostic and therapeutic strategies for small abdominal aortic aneurysms.Nature reviews. Cardiology · 2011Review
- Emerging diagnostic and therapeutic molecular imaging applications in vascular disease.Vascular medicine (London, England) · 2011Review
- Molecular imaging of coronary atherosclerosis and myocardial infarction: considerations for the bench and perspectives for the clinic.Circulation research · 2011Review
- Cardiovascular molecular imaging: focus on clinical translation.Circulation · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 1 country.
Funding
Abstract
objectiveAtherosclerotic plaque rupture leads to acute thrombus formation and may trigger serious clinical events such as myocardial infarction or stroke. Therefore, it would be valuable to identify atherothrombosis and vulnerable plaques before the onset of such clinical events. We sought to determine whether the noninvasive in vivo visualization of activated platelets was effective when using a target-specific MRI contrast agent to identify thrombi, hallmarks of vulnerable or high-risk atherosclerotic plaques. METHODS AND
resultsInflammatory thrombi were induced in mice via topical application of arachidonic acid on the carotid. Thrombus formation was imaged with intravital fluorescence microscopy and molecular MRI. To accomplish the latter, a paramagnetic contrast agent (P975) that targets the glycoprotein alpha(IIb)beta(3), expressed on activated platelets, was investigated. The specificity of P975 for activated platelets was studied in vitro. In vivo, high spatial-resolution MRI was performed at baseline and longitudinally over 2 hours after injecting P975 or a nonspecific agent. The contralateral carotid, a sham surgery group, and a competitive inhibition experiment served as controls. P975 showed a good affinity for activated platelets, with an IC(50) (concentration of dose that produces 50% inhibition) value of 2.6 micromol/L. In thrombosed animals, P975 produced an immediate and sustained increase in MRI signal, whereas none of the control groups revealed any significant increase in MRI signal 2 hours after injection. More important, the competitive inhibition experiment with an alpha(IIb)beta(3) antagonist suppressed the MRI signal enhancement, which is indicative for the specificity of P975 for the activated platelets.
conclusionsP975 allowed in vivo target-specific noninvasive MRI of activated platelets.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.