ArticleApplied and environmental microbiology2010
Detection and quantification of functional genes of cellulose- degrading, fermentative, and sulfate-reducing bacteria and methanogenic archaea.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 146 citations in OpenAlex.
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- Meso- and thermophilic posttreatment of press water coming from a thermophilic municipal solid waste digester.Biotechnology and bioengineering · 2024Article
- MCRL: using a reference library to compress a metagenome into a non-redundant list of sequences, considering viruses as a case study.Bioinformatics (Oxford, England) · 2022Article
- Microbial Contamination of Photographic and Cinematographic Materials in Archival Funds in the Czech Republic.Microorganisms · 2022Article
- Screening and identification of cellulose-degrading bacteria from soil and leaves at Kerman province, Iran.Archives of microbiology · 2021Article
- Crab shell amendments enhance the abundance and diversity of key microbial groups in sulfate-reducing columns treating acid mine drainage.Applied microbiology and biotechnology · 2020Article
- A Novel Primer Mixture for GH48 Genes: Quantification and Identification of Truly Cellulolytic Bacteria in Biogas Fermenters.Microorganisms · 2020Article
- Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.Applied microbiology and biotechnology · 2020Article
- Diversity of tryptophan halogenases in sponges of the genus Aplysina.FEMS microbiology ecology · 2019Article
- Acetate promotes microbial reductive debromination of tetrabromobisphenol A during the startup phase of anaerobic wastewater sludge bioreactors.The Science of the total environment · 2019Article
- Non-negligible greenhouse gases from urban sewer system.Biotechnology for biofuels · 2019Article
- Microbial Degradation of Cellulosic Material and Gas Generation: Implications for the Management of Low- and Intermediate-Level Radioactive Waste.Frontiers in microbiology · 2019Article
- Lignocellulosic biowastes as carrier material and slow release electron donor for sulphidogenesis of wastewater in an inverse fluidized bed bioreactor.Environmental science and pollution research international · 2018Article
- Assessing the colonic microbiome, hydrogenogenic and hydrogenotrophic genes, transit and breath methane in constipation.Neurogastroenterology and motility · 2017Article
- Microbial Abundances Predict Methane and Nitrous Oxide Fluxes from a Windrow Composting System.Frontiers in microbiology · 2017Article
- Article
- Archaeal Distribution in Moonmilk Deposits from Alpine Caves and Their Ecophysiological Potential.Microbial ecology · 2016Article
- Characterization and Adaptation of Anaerobic Sludge Microbial Communities Exposed to Tetrabromobisphenol A.PloS one · 2016Article
- The microbial community structure in industrial biogas plants influences the degradation rate of straw and cellulose in batch tests.Biotechnology for biofuels · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellulose degradation, fermentation, sulfate reduction, and methanogenesis are microbial processes that coexist in a variety of natural and engineered anaerobic environments. Compared to the study of 16S rRNA genes, the study of the genes encoding the enzymes responsible for these phylogenetically diverse functions is advantageous because it provides direct functional information. However, no methods are available for the broad quantification of these genes from uncultured microbes characteristic of complex environments. In this study, consensus degenerate hybrid oligonucleotide primers were designed and validated to amplify both sequenced and unsequenced glycoside hydrolase genes of cellulose-degrading bacteria, hydA genes of fermentative bacteria, dsrA genes of sulfate-reducing bacteria, and mcrA genes of methanogenic archaea. Specificity was verified in silico and by cloning and sequencing of PCR products obtained from an environmental sample characterized by the target functions. The primer pairs were further adapted to quantitative PCR (Q-PCR), and the method was demonstrated on samples obtained from two sulfate-reducing bioreactors treating mine drainage, one lignocellulose based and the other ethanol fed. As expected, the Q-PCR analysis revealed that the lignocellulose-based bioreactor contained higher numbers of cellulose degraders, fermenters, and methanogens, while the ethanol-fed bioreactor was enriched in sulfate reducers. The suite of primers developed represents a significant advance over prior work, which, for the most part, has targeted only pure cultures or has suffered from low specificity. Furthermore, ensuring the suitability of the primers for Q-PCR provided broad quantitative access to genes that drive critical anaerobic catalytic processes.
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