ArticleApplied and environmental microbiology2010
Identification of ciliate grazers of autotrophic bacteria in ammonia-oxidizing activated sludge by RNA stable isotope probing.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Predation by a ciliate community mediates temperature and nutrient effects on a peatland prey prokaryotic community.mSphere · 2025Article
- Active predation, phylogenetic diversity, and global prevalence of myxobacteria in wastewater treatment plants.The ISME journal · 2023Article
- Protist Predation Influences the Temperature Response of Bacterial Communities.Frontiers in microbiology · 2022Article
- DNA- and RNA-SIP RevealmBio · 2019Article
- Patterns of protist diversity associated with raw sewage in New York City.The ISME journal · 2019Article
- Community dynamics and functional characteristics of naphthalene-degrading populations in contaminated surface sediments and hypoxic/anoxic groundwater.Environmental microbiology · 2018Article
- An 18S rRNA Workflow for Characterizing Protists in Sewage, with a Focus on Zoonotic Trichomonads.Microbial ecology · 2017Article
- Eukaryotic Community Shift in Response to Organic Loading Rate of an Aerobic Trickling Filter (Down-Flow Hanging Sponge Reactor) Treating Domestic Sewage.Microbial ecology · 2017Article
- Eukaryotic molecular diversity at different steps of the wastewater treatment plant process reveals more phylogenetic novel lineages.World journal of microbiology & biotechnology · 2017Article
- Molecular diversity of eukaryotes in municipal wastewater treatment processes as revealed by 18S rRNA gene analysis.Microbes and environments · 2014Article
- Monitoring the effects of different conservation treatments on paper-infecting fungi.International biodeterioration & biodegradation · 2013Article
- Interactions of nitrifying bacteria and heterotrophs: identification of a Micavibrio-like putative predator of Nitrospira spp.Applied and environmental microbiology · 2013Article
- Metagenomic and metabolic profiling of nonlithifying and lithifying stromatolitic mats of Highborne Cay, The Bahamas.PloS one · 2012Article
- Development of a fatty acid and RNA stable isotope probing-based method for tracking protist grazing on bacteria in wastewater.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is well understood that protozoa play a major role in controlling bacterial biomass and regulating nutrient cycling in the environment. Little is known, however, about the movement of carbon from specific reduced substrates, through functional groups of bacteria, to particular clades of protozoa. In this study we first identified the active protozoan phylotypes present in activated sludge, via the construction of an rRNA-derived eukaryote clone library. Most of the sequences identified belonged to ciliates of the subclass Peritrichia and amoebae, confirming the dominance of surface-associated protozoa in the activated sludge environment. We then demonstrated that (13)C-labeled protozoan RNA can be retrieved from activated sludge amended with (13)C-labeled protozoa or (13)C-labeled Escherichia coli cells by using an RNA stable isotope probing (RNA-SIP) approach. Finally, we used RNA-SIP to track carbon from bicarbonate and acetate into protozoa under ammonia-oxidizing and denitrifying conditions, respectively. RNA-SIP analysis revealed that the peritrich ciliate Epistylis galea dominated the acquisition of carbon from bacteria with access to CO(2) under ammonia-oxidizing conditions, while there was no evidence of specific grazing on acetate consumers under denitrifying conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.