ArticleApplied and environmental microbiology2010
Requirement of a mitogen-activated protein kinase for appressorium formation and penetration of insect cuticle by the entomopathogenic fungus Beauveria bassiana.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 83 citations in OpenAlex.
- Beauveria bassiana acts as a beneficial endophyte in tea crops, modulating microbial communities and metabolic pathways to enhance plant growth.BMC plant biology · 2026Article
- MAPK Pathways Coordinate Stress Adaptation by Mobilizing Specialized Gene Modules in Entomopathogenic FungusJournal of fungi (Basel, Switzerland) · 2025Article
- An Integrative Multi-Source Evidence Framework for Prioritizing Virulence-Associated Pathways inGenes · 2025Article
- Analysis of the Effects ofJournal of fungi (Basel, Switzerland) · 2025Article
- Entomopathogenic fungi: insights into recent understanding.World journal of microbiology & biotechnology · 2025Review
- Beauveria bassiana transcriptomics reveal virulence-associated shifts during insect lipid assimilation.Applied microbiology and biotechnology · 2024Article
- Article
- A network of transcription factors in complex with a regulating cell cycle cyclin orchestrates fungal oxidative stress responses.BMC biology · 2024Article
- Altered Gene Expression of the ParasitoidInternational journal of molecular sciences · 2023Article
- Comparative transcriptome analysis to unveil genes affecting the host cuticle destruction in Metarhizium rileyi.Current genetics · 2023Article
- Expressing Parasitoid Venom Protein VRF1 in an EntomopathogenApplied and environmental microbiology · 2023Article
- Knockdown ofInternational journal of molecular sciences · 2023Article
- A life-and-death struggle: interaction of insects with entomopathogenic fungi across various infection stages.Frontiers in immunology · 2023Review
- Diversity and Function of Appressoria.Pathogens (Basel, Switzerland) · 2021Review
- Identification of a key G-protein coupled receptor in mediating appressorium formation and fungal virulence against insects.Science China. Life sciences · 2021Article
- The Insect Pathogens.Microbiology spectrum · 2017Review
- Entomopathogenicity and Biological Attributes of Himalayan Treasured Fungus Ophiocordyceps sinensis (Yarsagumba).Journal of fungi (Basel, Switzerland) · 2017Review
- Genome sequence and comparative analysis of clavicipitaceous insect-pathogenic fungus Aschersonia badia with Metarhizium spp.BMC genomics · 2016Article
- Genome Studies on Nematophagous and Entomogenous Fungi in China.Journal of fungi (Basel, Switzerland) · 2016Review
- Stress response signaling and virulence: insights from entomopathogenic fungi.Current genetics · 2015Review
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Beauveria bassiana is an important insect-pathogenic fungus that invades insects by direct penetration of the host cuticle. To delineate the molecular mechanisms involved in fungal infection, a mitogen-activated protein kinase (MAPK) gene, Bbmpk1, which encodes a YERK1 family MAPK was isolated and characterized. Targeted gene disruption of Bbmpk1 resulted in a complete loss of virulence when applied topically to host insects but did not affect growth of the fungus when conidia were injected directly into the hemocoel. Hyphae of the mutant strain growing in the insect hemocoel were unable to penetrate the cuticle growing outwards and consequently failed to sporulate on the cadaver surface. These data suggest that BbMPK1 is essential for penetration of the insect cuticle both from the outside and from the inside-out in order to escape and disperse from the host. Inactivation of BbMPK1 also caused a significant decrease in fungal adhesion to insect cuticles and eliminated their ability to form appressoria. In order to identify downstream genes regulated by BbMPK1, a suppressive subtractive hybridization (SSH) library was generated comparing mutant and wild-type transcripts isolated during appressorium formation. Thirty-one genes screened from the SSH library were determined to be expressed in the wild-type strain but either significantly reduced or not expressed in the mutant. Ten genes showed high or medium similarity to known protein encoding genes, including proteins involved in cell surface hydrophobicity, lipid metabolism, microtubule dynamics, mitochondrial electron transport, chromatin remodeling, transcription, rRNA processing, small nucleolar RNA accumulation, oxidation of aldehydes, translation, and likely other cellular processes.
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