ArticleEukaryotic cell2010
F-actin dynamics in Neurospora crassa.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed, 154 citations in OpenAlex.
- Article
- The actin module of endocytic internalization in Aspergillus nidulans: A critical role of the WISH/DIP/SPIN90 family protein Dip1.PLoS genetics · 2025Article
- Secretion and endocytosis in subapical cells support hyphal tip growth in the fungus Trichoderma reesei.Nature communications · 2025Article
- Natural Product Aloesin Significantly Inhibits Spore Germination and Appressorium Formation inMicroorganisms · 2023Article
- Live-cell imaging of septins and cell polarity proteins in the growing dikaryotic vegetative hypha of the model mushroom Coprinopsis cinerea.Scientific reports · 2023Article
- Complementation of an Eisosomal YeastJournal of fungi (Basel, Switzerland) · 2023Article
- Optimized fluorescent proteins for 4-color and photoconvertible live-cell imaging in Neurospora crassa.Fungal genetics and biology : FG & B · 2023Article
- Use of red, far-red, and near-infrared light in imaging of yeasts and filamentous fungi.Applied microbiology and biotechnology · 2022Review
- Synthetic Soil Aggregates: Bioprinted Habitats for High-Throughput Microbial Metaphenomics.Microorganisms · 2022Article
- Imaging the Actin Cytoskeleton in Live Budding Yeast Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Twinfilin regulates actin assembly and Hexagonal peroxisome 1 (Hex1) localization in the pathogenesis of rice blast fungus Magnaporthe oryzae.Molecular plant pathology · 2021Article
- Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes.Journal of fungi (Basel, Switzerland) · 2021Article
- Plasma membrane nano-organization specifies phosphoinositide effects on Rho-GTPases and actin dynamics in tobacco pollen tubes.The Plant cell · 2021Article
- Correlative Localization Analysis Between mRNA and Enhanced Green Fluorescence Protein-Fused Protein by a Single-Molecule FluorescenceFrontiers in fungal biology · 2021Article
- The Biology of Invasive Growth by the Rice Blast Fungus Magnaporthe oryzae.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi.AMB Express · 2020Article
- Autophagy machinery promotes the chaperone-mediated formation and compartmentalization of protein aggregates during appressorium development by the rice blast fungus.Molecular biology of the cell · 2020Article
- Magnaporthe oryzae fimbrin organizes actin networks in the hyphal tip during polar growth and pathogenesis.PLoS pathogens · 2020Article
- Single-Molecule FISH Reveals Subcellular Localization of α-Amylase and Actin mRNAs in the Filamentous FungusFrontiers in microbiology · 2020Article
- Expansion Microscopy for Cell Biology Analysis in Fungi.Frontiers in microbiology · 2020Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
This study demonstrates the utility of Lifeact for the investigation of actin dynamics in Neurospora crassa and also represents the first report of simultaneous live-cell imaging of the actin and microtubule cytoskeletons in filamentous fungi. Lifeact is a 17-amino-acid peptide derived from the nonessential Saccharomyces cerevisiae actin-binding protein Abp140p. Fused to green fluorescent protein (GFP) or red fluorescent protein (TagRFP), Lifeact allowed live-cell imaging of actin patches, cables, and rings in N. crassa without interfering with cellular functions. Actin cables and patches localized to sites of active growth during the establishment and maintenance of cell polarity in germ tubes and conidial anastomosis tubes (CATs). Recurrent phases of formation and retrograde movement of complex arrays of actin cables were observed at growing tips of germ tubes and CATs. Two populations of actin patches exhibiting slow and fast movement were distinguished, and rapid (1.2 microm/s) saltatory transport of patches along cables was observed. Actin cables accumulated and subsequently condensed into actin rings associated with septum formation. F-actin organization was markedly different in the tip regions of mature hyphae and in germ tubes. Only mature hyphae displayed a subapical collar of actin patches and a concentration of F-actin within the core of the Spitzenkörper. Coexpression of Lifeact-TagRFP and beta-tubulin-GFP revealed distinct but interrelated localization patterns of F-actin and microtubules during the initiation and maintenance of tip growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.