ArticleJournal of bacteriology2010
Increased expression of the type IV secretion system in piliated Neisseria gonorrhoeae variants.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Phenotypic Characterization ofAntibiotics (Basel, Switzerland) · 2025Article
- Pilin antigenic variants impact gonococcal lifestyle and antibiotic tolerance by modulating interbacterial forces.PLoS biology · 2025Article
- Genetic Manipulation of Neisseria gonorrhoeae and Commensal Neisseria Species.Current protocols · 2024Article
- SCCBiofilm · 2024Article
- DNA Transport through the Dynamic Type IV Secretion System.Infection and immunity · 2023Review
- The Neisseria gonorrhoeae type IV pilus promotes resistance to hydrogen peroxide- and LL-37-mediated killing by modulating the availability of intracellular, labile iron.PLoS pathogens · 2022Article
- Transcriptional and Translational Responsiveness of the Neisseria gonorrhoeae Type IV Secretion System to Conditions of Host Infections.Infection and immunity · 2021Article
- High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation inPathogens (Basel, Switzerland) · 2019Article
- DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island.Nucleic acids research · 2019Article
- Genome-wide screen for genes involved in eDNA release during biofilm formation byProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Mechanisms and Regulation of Extracellular DNA Release and Its Biological Roles in Microbial Communities.Frontiers in microbiology · 2017Review
- Neisseria gonorrhoeae Crippled Its Peptidoglycan Fragment Permease To Facilitate Toxic Peptidoglycan Monomer Release.Journal of bacteriology · 2016Article
- Targeted mutagenesis of intergenic regions in the Neisseria gonorrhoeae gonococcal genetic island reveals multiple regulatory mechanisms controlling type IV secretion.Molecular microbiology · 2015Article
- Mobile DNA in the pathogenicMicrobiology spectrum · 2015Article
- TraK and TraB are conserved outer membrane proteins of the Neisseria gonorrhoeae Type IV secretion system and are expressed at low levels in wild-type cells.Journal of bacteriology · 2014Article
- Article
- Prevalence and detailed mapping of the gonococcal genetic island in Neisseria meningitidis.Journal of bacteriology · 2012Article
- New complementation constructs for inducible and constitutive gene expression in Neisseria gonorrhoeae and Neisseria meningitidis.Applied and environmental microbiology · 2012Article
- Assembly and mechanisms of bacterial type IV secretion machines.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Characterization of the single stranded DNA binding protein SsbB encoded in the Gonoccocal Genetic Island.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Neisseria gonorrhoeae produces a type IV secretion system that secretes chromosomal DNA. The secreted DNA is active in the transformation of other gonococci in the population and may act to transfer antibiotic resistance genes and variant alleles for surface antigens, as well as other genes. We observed that gonococcal variants that produced type IV pili secreted more DNA than variants that were nonpiliated, suggesting that the process may be regulated. Using microarray analysis, we found that a piliated strain showed increased expression of the gene for the putative type IV secretion coupling protein TraD, whereas a nonpiliated variant showed increased expression of genes for transcriptional and translational machinery, consistent with its higher growth rate compared to that of the piliated strain. These results suggested that type IV secretion might be controlled by either traD expression or growth rate. A mutant with a deletion in traD was found to be deficient in DNA secretion. Further mutation and complementation analysis indicated that traD is transcriptionally and translationally coupled to traI, which encodes the type IV secretion relaxase. We were able to increase DNA secretion in a nonpiliated strain by inserting a gene cassette with a strong promoter to drive the expression of the putative operon containing traI and traD. Together, these data suggest a model in which the type IV secretion system apparatus is made constitutively, while its activity is controlled through regulation of traD and traI.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.