ArticleThe Plant cell2010
The Arabidopsis rcn1-1 mutation impairs dephosphorylation of Phot2, resulting in enhanced blue light responses.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 74 citations in OpenAlex.
- Blue light receptor phot2 collaborates with NRL-30 to negatively regulate immunity by reducing mitochondrial protein PRXIIF stability in potato.Molecular horticulture · 2026Article
- The Microtubule-Associated Protein CsTON2 Interacts With CsTRM5 and CsSUN to Regulate Fruit Shape Development in Cucumber.Plant biotechnology journal · 2026Article
- Reversible phosphorylation of NPH3/RPT2-like proteins regulates phototropin receptor signaling.The Plant cell · 2026Article
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- Hydrogen peroxide mediates high-intensity blue light-induced hypocotyl phototropism of cotton seedlings.Stress biology · 2023Article
- The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants.Stress biology · 2022Review
- SPEECHLESS and MUTE Mediate Feedback Regulation of Signal Transduction during Stomatal Development.Plants (Basel, Switzerland) · 2021Review
- Chloroplasts in C3 grasses move in response to blue-light.Plant cell reports · 2020Article
- Proteomics and phosphoproteomics revealed molecular networks of stomatal immune responses.Planta · 2020Article
- Protein phosphatase 2A promotes stomatal development by stabilizing SPEECHLESS inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Chloroplast avoidance movement: a novel paradigm of ROS signalling.Photosynthesis research · 2020Review
- The continuing arc toward phototropic enlightenment.Journal of experimental botany · 2020Article
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- PROTEIN PHOSHATASE 2A B'Plant physiology · 2018Article
- Plant pathogen effector utilizes host susceptibility factor NRL1 to degrade the immune regulator SWAP70.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
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- Blue light-excited LOV1 and LOV2 domains cooperatively regulate the kinase activity of full-length phototropin2 fromThe Journal of biological chemistry · 2018Article
- Involvement of PP6-type protein phosphatase in hypocotyl phototropism in Arabidopsis seedlings.Plant signaling & behavior · 2018Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phototropins (phot) sense blue light through the two N-terminal chromophore binding LOV domains and activate the C-terminal kinase domain. The resulting phototropin autophosphorylation is essential for biological activity. We identified the A1 subunit of Ser/Thr protein phosphatase 2A (PP2A) as interacting with full-length phot2 in yeast and also interacting with phot2 in an in vitro protein binding assay. Phenotypic characterizations of a phot1-5 rcn1-1 (for root curling in n-naphthylphthalamic acid1) double mutant, in which phot2 is the only functional phototropin and PP2A activity is reduced, showed enhanced phototropic sensitivity and enhanced blue light-induced stomatal opening, suggesting that PP2A activity is involved in regulating phot2 function. When treated with cantharidin, a chemical inhibitor of PP2A, the phot1-5 mutant exhibited enhanced phot2-mediated phototropic responses like those of the phot1-5 rcn1-1 double mutant. Immunoblot analysis to examine phot2 endogenous phosphorylation levels and in vitro phosphorylation assays of phot2 extracted from plants during dark recovery from blue light exposure confirmed that phot2 is more slowly dephosphorylated in the reduced PP2A activity background than in the wild-type PP2A background, suggesting that phosphorylated phot2 is a substrate of PP2A activity. While reduced PP2A activity enhanced the activity of phot2, it did not enhance either phot1 dephosphorylation or the activity of phot1 in mediating phototropism or stomatal opening.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.