ArticleThe Journal of membrane biology2010
A simple method for the reconstitution of membrane proteins into giant unilamellar vesicles.
Article in The Journal of membrane biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Spatially regulated mRNA translation enables functional membrane protein integration in synthetic cells.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Formation of Cell-Sized Liposomes Incorporating a β-Barrel-Structured Porin through Rehydration of a Phospholipid-Membrane Protein Dried Film.ACS omega · 2024Article
- Review
- Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes.International journal of molecular sciences · 2023Review
- Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter.Scientific reports · 2021Article
- Single Cell-like Systems Reveal Active Unidirectional and Light-Controlled Transport by Nanomachineries.ACS nano · 2021Article
- Patch-Clamp Recordings of the KcsA KMethods in molecular biology (Clifton, N.J.) · 2018Article
- Membrane protein reconstitution into giant unilamellar vesicles: a review on current techniques.European biophysics journal : EBJ · 2017Review
- A modular platform for one-step assembly of multi-component membrane systems by fusion of charged proteoliposomes.Nature communications · 2016Article
- Intracellular trafficking of the pyridoxal cofactor. Implications for health and metabolic disease.Archives of biochemistry and biophysics · 2016Review
- Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system.Protein expression and purification · 2016Article
- Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Local mitochondrial-endolysosomal microfusion cleaves voltage-dependent anion channel 1 to promote survival in hypoxia.Molecular and cellular biology · 2015Article
- Hydrogel-assisted functional reconstitution of human P-glycoprotein (ABCB1) in giant liposomes.Biochimica et biophysica acta · 2015Article
- Direct transmembrane interaction between actin and the pore-competent, cholesterol-dependent cytolysin pneumolysin.Journal of molecular biology · 2013Article
- Article
- Exosome mimetics: a novel class of drug delivery systems.International journal of nanomedicine · 2012Review
- Functional reconstitution of a voltage-gated potassium channel in giant unilamellar vesicles.PloS one · 2011Article
- Mechanical properties of lipid bilayers and regulation of mechanosensitive function: from biological to biomimetic channels.Channels (Austin, Tex.)Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple method for the reconstitution of membrane protein from submicron proteoliposomes into giant unilamellar vesicles (GUVs) is presented here: This method does not require detergents, fusion peptides or a dehydration step of the membrane protein solution. In a first step, GUVs of lipids were formed by electroformation, purified and concentrated; and in a second step, the concentrated GUV solution was added to a small volume of vesicles or proteoliposomes. Material transfer from submicron vesicles and proteoliposomes to GUVs occurred spontaneously and was characterized with fluorescent microscopy and patch-clamp recordings. As a functional test, the voltage-dependent, anion-selective channel protein was reconstituted into GUVs, and its electrophysiological activity was monitored with the patch clamp. This method is versatile since it is independent of the presence of the protein, as demonstrated by the fusion of fluorescently labeled submicron vesicles and proteoliposomes with GUVs.
Indexed as
Identifiers
20135103What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.