ArticleNature protocols2010
Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
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- Transcriptomic and de novo proteomic analyses of organotypic entorhino-hippocampal tissue cultures reveal changes in metabolic and signaling regulators in TTX-induced synaptic plasticity.Molecular brain · 2024Article
- Preclinical translational platform of neuroinflammatory disease biology relevant to neurodegenerative disease.Journal of neuroinflammation · 2024Article
- Optimized Protocol for Proportionate CNS Cell Retrieval as a Versatile Platform for Cellular and Molecular Phenomapping in Aging and Neurodegeneration.International journal of molecular sciences · 2022Article
- A culture model for the assessment of phenylalanine neurotoxicity in phenylketonuria.In vitro models · 2022Article
- Heat-Shock Induces Granule Cell Dispersion and Microgliosis in Hippocampal Slice Cultures.Frontiers in cell and developmental biology · 2021Article
- Nystatin Regulates Axonal Extension and Regeneration by Modifying the Levels of Nitric Oxide.Frontiers in molecular neuroscience · 2020Article
- Pharmacological enhancement ofScience translational medicine · 2019Article
- Cholesterol Depletion Regulates Axonal Growth and Enhances Central and Peripheral Nerve Regeneration.Frontiers in cellular neuroscience · 2019Article
- Human UCB-MSCs treatment upon intraventricular hemorrhage contributes to attenuate hippocampal neuron loss and circuit damage through BDNF-CREB signaling.Stem cell research & therapy · 2018Article
- New functions of Semaphorin 3E and its receptor PlexinD1 during developing and adult hippocampal formation.Scientific reports · 2018Article
- The Use ofFrontiers in veterinary science · 2018Review
- Bioenergetic state regulates innate inflammatory responses through the transcriptional co-repressor CtBP.Nature communications · 2017Article
- Article
- Organotypic Spinal Cord Culture: a Proper Platform for the Functional Screening.Molecular neurobiology · 2016Review
- p75 regulates Purkinje cell firing by modulating SK channel activity through Rac1.The Journal of biological chemistry · 2014Article
- Capture of activity-induced ultrastructural changes at synapses by high-pressure freezing of brain tissue.Nature protocols · 2014Article
- Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue.International journal of molecular sciences · 2013Article
- Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a method for using long-term organotypic slice co-cultures of the entorhino-hippocampal formation to analyze the axon-regenerative properties of a determined compound. The culture method is based on the membrane interphase method, which is easy to perform and is generally reproducible. The degree of axonal regeneration after treatment in lesioned cultures can be seen directly using green fluorescent protein (GFP) transgenic mice or by axon tracing and histological methods. Possible changes in cell morphology after pharmacological treatment can be determined easily by focal in vitro electroporation. The well-preserved cytoarchitectonics in the co-culture facilitate the analysis of identified cells or regenerating axons. The protocol takes up to a month.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.