ArticleProceedings of the National Academy of Sciences of the United States of America2010
T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 70 citations in OpenAlex.
- Phylogenomic framework and virulence gene boundaries of emerging Shiga toxin-producingVirulence · 2026Article
- Phage host range: determinants, dynamics and applications.Nature reviews. Microbiology · 2026Review
- Liquid-liquid phase separation and a phage-encoded inhibitor cooperatively drive transcriptional transition during phage SPO1 infection.Nucleic acids research · 2026Article
- Pseudomonas aeruginosa as a model bacterium in antiphage defense research.FEMS microbiology reviews · 2025Review
- Membrane platform protein PulF of themBio · 2024Article
- How to Shut Down Transcription in Archaea during Virus Infection.Microorganisms · 2022Review
- RNA Management During T7 Infection.PHAGE (New Rochelle, N.Y.) · 2022Article
- Bacteriophage protein Gp46 is a cross-species inhibitor of nucleoid-associated HU proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Phage-EncodedViruses · 2020Article
- NovelViruses · 2020Article
- Structural basis of transcription inhibition by the DNA mimic protein Ocr of bacteriophage T7.eLife · 2020Article
- Constrained Combinatorial Libraries of Gp2 Proteins Enhance Discovery of PD-L1 Binders.ACS combinatorial science · 2018Article
- T7 phage factor required for managing RpoS inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Engineered Charge Redistribution of Gp2 Proteins through Guided Diversity for Improved PET Imaging of Epidermal Growth Factor Receptor.Bioconjugate chemistry · 2018Article
- Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display.Microbial cell factories · 2018Review
- Conformational heterogeneity and bubble dynamics in single bacterial transcription initiation complexes.Nucleic acids research · 2018Article
- Phenotypic consequences of RNA polymerase dysregulation in Escherichia coli.Nucleic acids research · 2017Article
- Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein.Nucleic acids research · 2017Article
- Mechanism of duplex DNA destabilization by RNA-guided Cas9 nuclease during target interrogation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The bacterial enhancer-dependent RNA polymerase.The Biochemical journal · 2016Review
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12 authors at 4 institutions in 3 countries.
Funding
Abstract
Infection of Escherichia coli by the T7 phage leads to rapid and selective inhibition of the host RNA polymerase (RNAP)--a multi-subunit enzyme responsible for gene transcription--by a small ( approximately 7 kDa) phage-encoded protein called Gp2. Gp2 is also a potent inhibitor of E. coli RNAP in vitro. Here we describe the first atomic resolution structure of Gp2, which reveals a distinct run of surface-exposed negatively charged amino acid residues on one side of the molecule. Our comprehensive mutagenesis data reveal that two conserved arginine residues located on the opposite side of Gp2 are important for binding to and inhibition of RNAP. Based on a structural model of the Gp2-RNAP complex, we propose that inhibition of transcription by Gp2 involves prevention of RNAP-promoter DNA interactions required for stable DNA strand separation and maintenance of the "transcription bubble" near the transcription start site, an obligatory step in the formation of a transcriptionally competent promoter complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.