ArticleProceedings of the National Academy of Sciences of the United States of America2010
Synthetic design of strong promoters.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 132 citations in OpenAlex.
- Functional characterization of viral transcriptional regulators reveals extensive rewiring of host pathways.Nature communications · 2026Article
- Evaluating DNA Function Understanding in Genomic Language Models Using Evolutionarily Implausible Sequences.ACS synthetic biology · 2026Article
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- A New Conditional Transcription Factor for Use in Toxoplasma Gondii.Biotechnology journal · 2026Article
- AnFrontiers in genetics · 2025Article
- Direct testing of natural twister ribozymes from over a thousand organisms reveals a broad tolerance for structural imperfections.Nucleic acids research · 2024Article
- Designing Cell-Type-Specific Promoter Sequences Using Conservative Model-Based Optimization.bioRxiv : the preprint server for biology · 2024Article
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- Logical design of synthetic cis-regulatory DNA for genetic tracing of cell identities and state changes.Nature communications · 2024Article
- Cell factory engineering: Challenges and opportunities for synthetic biology applications.Biotechnology and bioengineering · 2023Review
- Systems Biology Approaches for the Improvement of Oncolytic Virus-Based Immunotherapies.Cancers · 2023Review
- Efficient Selection of Enhancers and Promoters from MIA PaCa-2 Pancreatic Cancer Cells by ChIP-lentiMPRA.International journal of molecular sciences · 2022Article
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- Deciphering the molecular basis of tissue-specific gene expression in plants: Can synthetic biology help?Current opinion in plant biology · 2022Review
- High throughput screen for the improvement of inducible promoters for tumor microenvironment cues.Scientific reports · 2022Article
- Oligo Pools as an Affordable Source of Synthetic DNA for Cost-Effective Library Construction in Protein- and Metabolic Pathway Engineering.Chembiochem : a European journal of chemical biology · 2022Review
- Engineering a synthesis-friendly serum responsive promoter for antibiotic selection of genomic integration in cell-based therapy.Biotechnology letters · 2022Article
- Creation of a synthesis-friendly inflammation-inducible promoter suitable for cell therapy.Integrative biology : quantitative biosciences from nano to macro · 2021Article
- Development of a rabies virus-based retrograde tracer with high trans-monosynaptic efficiency by reshuffling glycoprotein.Molecular brain · 2021Article
- Synthetic and systems biology principles in the design of programmable oncolytic virus immunotherapies for glioblastoma.Neurosurgical focus · 2021Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have taken a synthetic biology approach to the generation and screening of transcription factor binding sites for activity in human cells. All possible 10-mer DNA sequences were printed on microarrays as 100-mers containing 10 repeats of the same sequence in tandem, yielding an oligonucleotide library of 52,429 unique sequences. This library of potential enhancers was introduced into a retroviral vector and screened in multiple cell lines for the ability to activate GFP transcription from a minimal CMV promoter. With this method, we isolated 100 bp synthetic enhancer elements that were as potent at activating transcription as the WT CMV immediate early enhancer. The activity of the recovered elements was strongly dependent on the cell line in which they were recovered. None of the elements were capable of achieving the same levels of transcriptional enhancement across all tested cell lines as the CMV enhancer. A second screen, for enhancers capable of synergizing with the elements from the original screen, yielded compound enhancers that were capable of twofold greater enhancement activity than the CMV enhancer, with higher levels of activity than the original synthetic enhancer across multiple cell lines. These findings suggest that the 10-mer synthetic enhancer space is sufficiently rich to allow the creation of synthetic promoters of all strengths in most, if not all, cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.