ArticleProceedings of the National Academy of Sciences of the United States of America2010
Visualizing dynamic interaction between calmodulin and calmodulin-related kinases via a monitoring method in live mammalian cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- A co-overexpression strategy allows effective protein-protein binding affinities to be assessed as a function of concentration within live cells.bioRxiv : the preprint server for biology · 2026Article
- Let go or retain: role of calmodulin in orchestrating nuclear protein transport in eukaryotes.Plant cell reports · 2026Review
- Calcium signaling and postsynaptic density dynamics: the roles of calmodulin in synaptic protein regulation.Frontiers in molecular neuroscience · 2026Review
- Imaging the Binding Between Dasatinib and Its Target Protein in Living Cells Using an SLP Tag System on Intracellular Compartments.International journal of molecular sciences · 2025Article
- ReLo is a simple and rapid colocalization assay to identify and characterize direct protein-protein interactions.Nature communications · 2024Article
- Distribution of Signal Peptides in Microvesicles from Activated Macrophage Cells.International journal of molecular sciences · 2023Article
- Article
- NIMA-related kinase 9 regulates the phosphorylation of the essential myosin light chain in the heart.Nature communications · 2022Article
- Facile analysis of protein-protein interactions in living cells by enriched visualization of the p-body.BMB reports · 2018Article
- Surveying the landscape of optogenetic methods for detection of protein-protein interactions.Wiley interdisciplinary reviews. Systems biology and medicine · 2018Review
- Constitutive activation of CaMKKα signaling is sufficient but not necessary for mTORC1 activation and growth in mouse skeletal muscle.American journal of physiology. Endocrinology and metabolism · 2014Article
- An optimized optogenetic clustering tool for probing protein interaction and function.Nature communications · 2014Article
- Reversible protein inactivation by optogenetic trapping in cells.Nature methods · 2014Article
- Real-time single-molecule coimmunoprecipitation of weak protein-protein interactions.Nature protocols · 2013Article
- Article
- Setting Up a Bioluminescence Resonance Energy Transfer High throughput Screening Assay to Search for Protein/Protein Interaction Inhibitors in Mammalian Cells.Frontiers in endocrinology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new visualizing method was developed for monitoring protein-protein (P-P) interactions in live mammalian cells. P-P interactions are visualized by directing localization of a bait protein to endosomes. This method is sufficiently robust to analyze signal-dependent P-P interactions such as calcium-dependent protein interactions. We visualized interactions between activated calmodulin and calmodulin-binding proteins, and observed oscillatory interactions via time-lapse imaging. In addition, this new method can simultaneously monitor multiple P-P interactions in a single live cell, which allows comparison of interactions between several prey proteins and a single bait protein. We observed that CaMKK1 and CaMKIIalpha bind calmodulin with distinct binding affinities in live cell, which indicates that calcium signaling is fine-tuned by distinct activation patterns of CaM kinases. This method will enable investigation of cellular processes based on dynamic P-P interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.